Abstract
Todarodes pacificus is a commercially important squid species; however, there is little information available regarding the population genetics of this species and effective molecular markers are still unavailable. In the present study, 107 novel single nucleotide polymorphism (SNP) markers were developed using Genotyping-by-Sequencing (GBS). The observed and expected heterozygosities ranged from 0.0345 to 0.5862 and 0.0339–0.4994, respectively. The polymorphism information content ranged from 0.0333 to 0.3747. Among these SNPs, all loci have been confirmed to fit the Hardy–Weinberg equilibrium (p > 0.05). The novel SNPs developed in this study will be helpful for the genetic conservation and scientific management of Todarodes pacificus.
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Funding
This work is supported by the National Key R&D Program of China (Grant No. 2019YFD0901404), the National Natural Science Foundation of China (Grant no. 31702312) and the National Natural Science Foundation of China (Grant no. 41506184).
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XL and XH carried out most of the experiments; CW designed the methods and experiments, interpreted the results and finished the discussion. YL prepared the materials and partly worked on the experiments. HL was responsible for overall supervision, and participated in coordination. All authors read and approved the final manuscript.
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Experimental protocols involving live animals were approved by the Ethics Committee for the Use of Animal Subjects of Shanghai Ocean University.
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Li, X., Hu, X., Lu, H. et al. Development and validation of 107 SNP markers in Todarodes pacificus (Ommastrephidae). Conservation Genet Resour 13, 417–424 (2021). https://doi.org/10.1007/s12686-021-01227-2
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DOI: https://doi.org/10.1007/s12686-021-01227-2