Abstract
Varunid crabs Hemigrapsus sanguineus and Hemigrapsus takanoi are species native from intertidal coastal areas in North-western Pacific and have become invasive along the Atlantic European coasts. To gain insights into population genetic features, we developed and characterized 16 (H. sanguineus) and 11 (H. takanoi) novel polymorphic microsatellite loci from next-generation sequencing. The number of alleles ranged from 2 to 20 for H. sanguineus, and from 8 to 21 for H. takanoi. Expected heterozygosity ranged from 0.470 to 0.947 and from 0.313 to 0.781, with mean multilocus F is estimates suggesting rapid turnover of populations. Overall, these microsatellite markers showed very high levels of polymorphism that will facilitate population genetic studies devoted to track down the most likely sources of introduction.
References
Asakura A, Watanabe S (2005) Hemigrapsus takanoi, new species, a sibling species of the common Japanese intertidal crab H. penicillatus (Decapoda: Brachyura: Grapsoidea). J Crust Biol 25:279–292
Favre-Bac L, Godé C, Arnaud J-F (2014) Characterization of polymorphic microsatellite markers for the fine-leaved water-dropwort Oenanthe aquatica and the Gypsywort Lycopus europaeus, two farmland remnant wetland species. Conserv Genet Res 6:995–998
Gothland M, Dauvin J-C, Denis L, Dufossé F, Jobert S, Ovaert J, Pezy J-P, Tous Rius A, Spilmont N (2014) Biological traits explain the distribution and colonisation ability of the invasive shore crab Hemigrapsus takanoi. Estuar Costal Shelf Sci 142:41–49
Goudet J (1995) FSTAT (Version 1.2). A computer program to calculate F-statistics. J Hered 86:485–486
Malausa T, Gilles A, Meglécz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Délye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Malé P-J, Ferreira S, Martin J-F (2011) High-throughput microsatellite isolation through 454 GS-FLX titanium pyrosequencing of enriched DNA libraries. Mol Ecol Res 11:638–644
Acknowledgments
This work received financial support from the French program “EC2CO”.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Poux, C., Gothland, M., Holl, AC. et al. Highly polymorphic microsatellite markers in two species, the invasive shore crabs Hemigrapsus sanguineus and Hemigrapsus takanoi (Decapoda, Varunidae). Conservation Genet Resour 7, 569–572 (2015). https://doi.org/10.1007/s12686-015-0426-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-015-0426-6