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Characterization of polymorphic microsatellite markers for the fine-leaved water-Dropwort Oenanthe aquatica and the Gypsywort Lycopus europaeus, two farmland remnant wetland species

  • Microsatellite Letters
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Abstract

Oenanthe aquatica (Apiaceae) and Lycopus europaeus (Lamiaceae) are two hygrophilous plant species found in remnant wetland habitats in agricultural areas. O. aquatica is declining and protected and L. europaeus more common, while still restricted to remnant wet habitats. To gain insights into their population genetic structure, we developed and characterized 16 (O. aquatica) and 15 (L. europaeus) novel polymorphic microsatellite markers from next-generation sequencing. The number of alleles ranged from 1 to 14 for O. aquatica, and from 1 to 9 for L. europaeus. Expected heterozygosity ranged from 0.156 to 0.903 and from 0.333 to 0.749, with a mean multilocus F is estimate of 0.043 and 0.092 for O. aquatica and L. europaeus, respectively. Overall, these newly developed microsatellite markers showed high levels of polymorphism that will facilitate fine-scaled population genetic studies.

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References

  • Goudet J (1995) FSTAT (version 1.2). A computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Malausa T, Gilles A, Meglécz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Délye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Malé P-J, Ferreira S, Martin J-F (2011) High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNAlibraries. Mol Ecol Res 11:638–644

    Article  CAS  Google Scholar 

  • Zedler JB, Kercher S (2005) Wetland resources: status, trends, ecosystem services, and restorability. Annu Rev Environ Resour 30:39–74

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank A. Gareil and S. Mahé for laboratory assistance. This work was funded by the “Région Nord-Pas de Calais” (CORECOL project).

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Authors and Affiliations

  1. UMR CNRS 6553 ECOBIO, Université de Rennes 1, Campus de Beaulieu, 35042, Rennes Cedex, France

    Lisa Favre-Bac

  2. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8198, Université des Sciences et Technologies de Lille – Lille 1, 59655, Villeneuve d’Ascq Cedex, France

    Cécile Godé & Jean-François Arnaud

Authors
  1. Lisa Favre-Bac
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  2. Cécile Godé
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  3. Jean-François Arnaud
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Correspondence to Jean-François Arnaud.

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Favre-Bac, L., Godé, C. & Arnaud, JF. Characterization of polymorphic microsatellite markers for the fine-leaved water-Dropwort Oenanthe aquatica and the Gypsywort Lycopus europaeus, two farmland remnant wetland species. Conservation Genet Resour 6, 995–998 (2014). https://doi.org/10.1007/s12686-014-0267-8

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  • DOI: https://doi.org/10.1007/s12686-014-0267-8

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