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Development of 12 polymorphic microsatellite DNA markers for the kelp Ecklonia cava (Phaeophyceae, Laminariales)

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Abstract

Twelve polymorphic microsatellite markers were isolated from the kelp Ecklonia cava Kjellman (Phaeophyceae, Laminariales). The applicability of these markers was confirmed by genotyping of 24 individuals from a natural population in Japan. All 12 loci were polymorphic, with the number of alleles ranging from 2 to 11 per locus. The range of expected heterozygosities in the population was 0.187–0.867. These informative microsatellite markers will be useful in genetic studies of this species.

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Acknowledgments

We sincerely wish to thank Y. Tsuchiya, H. Abe, and A. Matsuo for their technical help. We also thank Y. Sasakura and D. Muraoka for their technical advice. This study was supported by the Mikimoto Fund for Marine Ecology. This is contribution No. 754 from the Shimoda Marine Research Center, University of Tsukuba.

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Authors and Affiliations

  1. Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka, 415-0025, Japan

    Tomoko Itou & Kazuo Inaba

  2. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiya-Machi, Aoba, Sendai, Miyagi, 981-8555, Japan

    Manami Kanno, Azusa Kamiyama, Akihiro Kijima & Masakazu N. Aoki

  3. Field Science Center, Graduate School of Agricultural Science, Tohoku University, 232-3, Yomogida, Naruko-Onsen, Osaki, Miyagi, 989-6711, Japan

    Yoshihisa Suyama

  4. Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8564, Japan

    Shingo X. Sakamoto

Authors
  1. Tomoko Itou
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  2. Manami Kanno
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  3. Yoshihisa Suyama
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  4. Azusa Kamiyama
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  5. Shingo X. Sakamoto
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  6. Akihiro Kijima
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  7. Kazuo Inaba
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  8. Masakazu N. Aoki
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Corresponding author

Correspondence to Masakazu N. Aoki.

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Itou, T., Kanno, M., Suyama, Y. et al. Development of 12 polymorphic microsatellite DNA markers for the kelp Ecklonia cava (Phaeophyceae, Laminariales). Conservation Genet Resour 4, 459–461 (2012). https://doi.org/10.1007/s12686-011-9574-5

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  • DOI: https://doi.org/10.1007/s12686-011-9574-5

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