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Isolation and characterization of microsatellite markers for the dusky smoothhound shark, Mustelus canis

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Abstract

A total of 32 nuclear-encoded microsatellites, including 15 polymorphic and 13 monomorphic microsatellites, isolated from an enriched genomic library of the triakid shark, Mustelus canis (dusky smoothhound), and four microsatellites previously isolated from two other triakid sharks were characterized in 91 individuals of M. canis. Polymorphic repeat motifs included 12 dinucleotide, two trinucleotide and one pentanucleotide repeat(s). Mustelus canis supports both commercial and recreational fisheries throughout its range in the western Atlantic Ocean from Maine (USA) to southern Argentina. The microsatellites characterized will be useful in studies of population structure of M. canis and other triakid sharks.

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Acknowledgments

We thank C. McCandless of the NMFS Apex Predators Program for providing tissue samples. Work was supported by the Saltonstall-Kennedy Program (Department of Commerce) under Award NA10NMF4270218. This paper is number 82 in the series ‘Genetic Studies in Marine Fishes’ and Contribution No. 196 of the Center for Biosystematics and Biodiversity at Texas A&M University.

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  1. Center for Biosystematics and Biodiversity Texas A&M University, College Station, TX, 77843-2258, USA

    Melissa Giresi, Mark A. Renshaw, David S. Portnoy & John R. Gold

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  1. Melissa Giresi
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  2. Mark A. Renshaw
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  3. David S. Portnoy
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  4. John R. Gold
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Correspondence to Melissa Giresi.

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Giresi, M., Renshaw, M.A., Portnoy, D.S. et al. Isolation and characterization of microsatellite markers for the dusky smoothhound shark, Mustelus canis . Conservation Genet Resour 4, 101–104 (2012). https://doi.org/10.1007/s12686-011-9484-6

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  • DOI: https://doi.org/10.1007/s12686-011-9484-6

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