Abstract
Twenty-one nuclear-encoded microsatellites were isolated from an enriched genomic DNA library of coney, Cephalopholis fulva, and characterized in both C. fulva and red hind, Epinephelus guttatus. The microsatellites include 16 dinucleotide repeats, two trinucleotide repeats, and three tetranucleotide repeats. An additional 11 microsatellites, isolated originally from an enriched genomic DNA library of E. guttatus, were characterized in both E. guttatus and C. fulva. Both grouper species support important commercial and recreational fisheries in the western Atlantic along the coasts of North, Central, and South America.
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Acknowledgments
We thank H. Rivera and W. Tobias for sampling in St. Croix and D. Matos-Caraballo and L. Rivera-Padilla for sampling in Puerto Rico. Work was supported by the Cooperative Research Program (CRP) of the US Department of Commerce (Grant NA08-NMF-4540400) and by the Texas AgriLIFE Research (Project H-6703). This paper is number 67 in the series ‘Genetic Studies in Marine Fishes’ and Contribution No. 167 of the Center for Biosystematics and Biodiversity at Texas A & M University.
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Renshaw, M.A., Portnoy, D.S. & Gold, J.R. PCR primers for nuclear-encoded microsatellites of the groupers Cephalopholis fulva (coney) and Epinephelus guttatus (red hind). Conserv Genet 11, 1197–1202 (2010). https://doi.org/10.1007/s10592-009-9918-9
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DOI: https://doi.org/10.1007/s10592-009-9918-9