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Identification and characterization of microsatellite marker in the tetraploid Juniperus tibetica Kom. using next generation sequencing

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Abstract

Juniperus tibetica Kom. is a tetraploid endemic tree species of the southern Tibetan Plateau. For this species ten microsatellite markers were developed using next generation sequencing. To characterize these loci a sample of 14 juniper individuals from seven populations covering large parts of the species’ distribution range was analyzed. All loci were polymorphic, ranging from two to 15 alleles per locus. Expected heterozygosity was estimated using the program TETRASAT, and ranged from 0.20 to 0.90 across loci. These markers will be used in ongoing population genetic studies to evaluate the potential genetic depauperation of the highly fragmented forest remains on the southern Tibetan Plateau and to propose strategies for conservation management.

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Acknowledgements

I would like to thank Tina Mengel for providing essential assistance with the lab work and Giovanni Vendramin and Sascha Liepelt for their valuable advice. Furthermore I would like to thank Liza Bukovnik and the Duke IGSP Sequencing Core Facility for their service. This research was supported by grants from the German Research Council (DFG Grants Mi 271-18 and Zi 698-6), and the German Academic Exchange Program (DAAD).

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  1. Conservation Biology, University of Marburg, Karl-von Frisch Strasse 8, 35032, Marburg, Germany

    Lars Opgenoorth

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  1. Lars Opgenoorth
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Correspondence to Lars Opgenoorth.

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Opgenoorth, L. Identification and characterization of microsatellite marker in the tetraploid Juniperus tibetica Kom. using next generation sequencing. Conservation Genet Resour 1, 253 (2009). https://doi.org/10.1007/s12686-009-9062-3

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  • DOI: https://doi.org/10.1007/s12686-009-9062-3

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