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Ethanol Production from Acid Pretreated Food Waste Hydrolysate Using Saccharomyces cerevisiae 74D694 and Optimizing the Process Using Response Surface Methodology

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Abstract

Ethanol production from acid pretreated food waste hydrolysate using immobilized Saccharomyces cerevisiae 74D694 was investigated under different conditions in a batch experiment. Ethanol yield was measured at different time intervals (38, 48, 72, 96 and 105 h) using different immobilized bead ratios (25:100, 30:100, 40:100, 50:100 and 54:100, w/v). Food waste was pretreated using dilute sulphuric acid and the hydrolysate was filtered. The dry food waste had an initial reducing sugar content of 46% (w/w). After dilute acid pretreatment, reducing sugar content increased to 62%. The present study utilized liquid hydrolysate for ethanol production. The process was optimized using central composite design (CCD) a statistical tool used for optimization in response surface methodology (RSM). RSM predicted a maximum ethanol yield of 0.044 g/g of soluble solid in liquid hydrolysate at 40 h fermentation time and immobilized bead ratio of 54:100 (w/v). An experiment was run at the optimal condition and an ethanol yield of 0.047 g/g of soluble solid in liquid hydrolysate was obtained. The predicted result was thus experimentally verified.

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Abbreviations

RSM:

Response surface methodology

CCD:

Central composite design

YPD:

Yeast peptone dextrose

ANOVA:

Analysis of variance

FID:

Flame ionization detector

CV:

Coefficient of variance

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Acknowledgements

This research was funded by Ministry of Human Resource and Development, Government of India, under FAST program.

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  1. Department of Civil Engineering, IIT Hyderabad, Kandi, 502285, India

    Marttin Paulraj Gundupalli & Debraj Bhattacharyya

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  1. Marttin Paulraj Gundupalli
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  2. Debraj Bhattacharyya
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Correspondence to Debraj Bhattacharyya.

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Gundupalli, M.P., Bhattacharyya, D. Ethanol Production from Acid Pretreated Food Waste Hydrolysate Using Saccharomyces cerevisiae 74D694 and Optimizing the Process Using Response Surface Methodology. Waste Biomass Valor 10, 701–708 (2019). https://doi.org/10.1007/s12649-017-0077-9

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  • DOI: https://doi.org/10.1007/s12649-017-0077-9

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