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Neurotrophic Effects of Growth/Differentiation Factor 5 in a Neuronal Cell Line

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Abstract

The neurotrophin growth/differentiation factor 5 (GDF5) is studied as a potential therapeutic agent for Parkinson’s disease as it is believed to play a role in the development and maintenance of the nigrostriatal system. Progress in understanding the effects of GDF5 on dopaminergic neurones has been hindered by the use of mixed cell populations derived from primary cultures or in vivo experiments, making it difficult to differentiate between direct and indirect effects of GDF5 treatment on neurones. In an attempt to establish an useful model to study the direct neuronal influence of GDF5, we have characterised the effects of GDF5 on a human neuronal cell line, SH-SY5Y. Our results show that GDF5 has the capability to promote neuronal but not dopaminergic differentiation. We also show that it promotes neuronal survival in vitro following a 6-hydroxydopamine insult. Our results show that application of GDF5 to SH-SY5Y cultures induces the SMAD pathway which could potentially be implicated in the intracellular transmission of GDF5’s neurotrophic effects. Overall, our study shows that the SH-SY5Y neuroblastoma cell line provides an excellent neuronal model to study the neurotrophic effects of GDF5.

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Acknowledgments

The authors would like to thank Dr Jens Pohl from Biopharm GmbH for the generous gift of GDF5, Dr Gerard O’Keeffe from University College Cork for his comments, and the Higher Education Authority (Ireland) for funding through the PRTLI3 programme.

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Authors and Affiliations

  1. Department of Anatomy and Neuroscience, University College Cork, College Road, Cork, Ireland

    André Toulouse, Grace C. Collins & Aideen M. Sullivan

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  1. André Toulouse
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  2. Grace C. Collins
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  3. Aideen M. Sullivan
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Correspondence to André Toulouse.

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Toulouse, A., Collins, G.C. & Sullivan, A.M. Neurotrophic Effects of Growth/Differentiation Factor 5 in a Neuronal Cell Line. Neurotox Res 21, 256–265 (2012). https://doi.org/10.1007/s12640-011-9266-7

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  • DOI: https://doi.org/10.1007/s12640-011-9266-7

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