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Characterization of Exopolysaccharides (EPSs) Obtained from Ligilactobacillus salivarius Strains and Investigation at the Prebiotic Potential as an Alternative to Plant Prebiotics at Poultry

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Abstract

In this study, it was aimed to reveal the potential of using exopolysaccharides (EPS) obtained from Ligilactobacillus salivarius as a prebiotic that regulates chicken intestinal microbiota. Characterization of EPS obtained from L. salivarius BIS312 (EPSBIS312) and BIS722 (EPSBIS722) strains was demonstrated by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and size-exclusion chromatography (SEC) analyses. It was determined that the molecular weight of both EPS is in the range of 104–106 Daltons, and there are 4 types of monomers in their structure. Anti-biofilm and anti-quorum sensing effects of EPSBIS312 and EPSBIS722 were determined. EPSBIS312 and EPSBIS722 showed a strong anti-biofilm effect on Enterococcus faecalis ATCC 29212, Staphylococcus aureus EB-1, and Escherichia coli ATCC 11229. The anti-quorum sensing study revealed that the EPSBIS722 had a higher effect than the EPSBIS312. The effect of different concentrations of EPS (2.5%, 5%, 10%) on lactobacilli growth stimulator (LGS) was evaluated. The highest LGS was promoted at 10% concentration while the lowest LGS was promoted at 2.5% concentration by EPSBIS722. In addition, adhesion abilities of EPSBIS312 and EPSBIS722 in HT-29 colorectal adenocarcinoma cell line were tested. EPSs significantly increased the ability to adhere to HT-29 cells. The characterized EPSs may be an alternative to plant prebiotics such as inulin at poultry.

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The data that support the findings of this study are available from the corresponding author on request.

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Acknowledgements

The authors thanks Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (TAGEM/15/AR-GE/40) for financial supporting.

Funding

The authors would like to thank Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies for funding the TAGEM/15/AR-GE/40 coded project including this study.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Gazi University, 06500, Ankara, Turkey

    Seda Bikric, Belma Aslim, İlknur Dincer & Zehranur Yuksekdag

  2. Faculty of Science, Department of Biology, Süleyman Demirel University, Isparta, Turkey

    Seyhan Ulusoy

  3. Faculty of Science, Department of Chemistry, Gazi University, Ankara, Turkey

    Serkan Yavuz

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  1. Seda Bikric
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Correspondence to Zehranur Yuksekdag.

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Bikric, S., Aslim, B., Dincer, İ. et al. Characterization of Exopolysaccharides (EPSs) Obtained from Ligilactobacillus salivarius Strains and Investigation at the Prebiotic Potential as an Alternative to Plant Prebiotics at Poultry. Probiotics & Antimicro. Prot. 14, 49–59 (2022). https://doi.org/10.1007/s12602-021-09790-8

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