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Structural analysis and prebiotic activity of exopolysaccharide produced by probiotic strain Bifidobacterium bifidum EPS DA-LAIM

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Abstract

Exopolysaccharide (EPS)-producing Bifidobacterium bifidum EPS DA-LAIM was isolated from healthy human feces, the structure of purified EPS from the strain was analyzed, and its prebiotic activity was evaluated. The EPS from B. bifidum EPS DA-LAIM is a glucomannan-type heteropolysaccharide with a molecular weight of 407–1007 kDa, and its structure comprises 2-mannosyl, 6-mannosyl, and 2,6-mannosyl residues. The purified EPS promoted the growth of representative lactic acid bacteria and bifidobacterial strains. Bifidobacterium bifidum EPS DA-LAIM increased nitric oxide production in RAW 264.7 macrophage cells, indicating its immunostimulatory activity. Bifidobacterium bifidum EPS DA-LAIM also exhibited high gastrointestinal tract tolerance, gut adhesion ability, and antioxidant activity. These results suggest that EPS from B. bifidum EPS DA-LAIM is a potentially useful prebiotic material, and B. bifidum EPS DA-LAIM could be applied as a probiotic candidate.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120, Republic of Korea

    Min Joo Kang, Huijin Jeong, Suin Kim, Jaein Shin, Youngbo Song, Byung-Hoo Lee & Young-Seo Park

  2. Research Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin, 17073, Republic of Korea

    Hyoung-Geun Park, Tae-Ho Lee, Hai-Hua Jiang, Young-Sun Han, Bong-Gyeong Lee, Ho-Jin Lee & Min-Ju Park

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  1. Min Joo Kang
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Kang, M.J., Jeong, H., Kim, S. et al. Structural analysis and prebiotic activity of exopolysaccharide produced by probiotic strain Bifidobacterium bifidum EPS DA-LAIM. Food Sci Biotechnol 32, 517–529 (2023). https://doi.org/10.1007/s10068-022-01213-w

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