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Plant Growth Enhancement, Disease Resistance, and Elemental Modulatory Effects of Plant Probiotic Endophytic Bacillus sp. Fcl1

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Abstract

Endophytic bacteria have already been studied for their beneficial support to plants to manage both biotic and abiotic stress through an array of well-established mechanisms. They have either direct or indirect impact on mobilizing diverse nutrients and elements from soil to plants. However, detailed insight into the fine-tuning of plant elemental composition by associated microorganism is very limited. In this study, endophytic Bacillus Fcl1 characterized from the rhizome of Curcuma longa was found to have broad range of plant growth-promoting and biocontrol mechanisms. The organism was found to have indole acetic acid and 1-aminocyclopropane-1-carboxylate deaminase production properties along with nitrogen fixation. The Bacillus Fcl1 could also inhibit diverse phytopathogens as confirmed by dual culture and well diffusion. By LC-MS/MS analysis, chemical basis of its antifungal activity has been proved to be due to the production of iturin A and a blend of surfactin compounds. Moreover, the organism was found to induce both plant growth and disease resistance in vivo in model plant system. Because of these experimentally demonstrated multiple plant probiotic features, Bacillus Fcl1 was selected as a candidate organism to study its role in modulation of plant elemental composition. ICP-MS analysis of Bacillus Fcl1-treated plants provided insight into relation of bacterial interaction with elemental composition of plants.

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Acknowledgments

The authors acknowledge Prof. C. T. Aravindakumar, Hon. Director, and Mr. Dineep D., Scientific Assistant of the Inter-University Instrumentation Centre, Mahatma Gandhi University, Kottayam, for LC-MS and LC-MS/ MS analysis.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India

    Aswathy Jayakumar & E. K. Radhakrishnan

  2. Sree Narayana Arts and Science College, Kumarakom, India

    Arathy Krishna

  3. School of Environmental Sciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India

    Mahesh Mohan

  4. Department of Biotechnology, SAS SNDP YOGAM, College, Konni, India

    Indu C. Nair

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  1. Aswathy Jayakumar
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  5. E. K. Radhakrishnan
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Correspondence to E. K. Radhakrishnan.

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Jayakumar, A., Krishna, A., Mohan, M. et al. Plant Growth Enhancement, Disease Resistance, and Elemental Modulatory Effects of Plant Probiotic Endophytic Bacillus sp. Fcl1. Probiotics & Antimicro. Prot. 11, 526–534 (2019). https://doi.org/10.1007/s12602-018-9417-8

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