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Suppression mechanisms on proliferation of glioma U251 cells by FePt nanoparticles through intracellular oxidative stress

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摘要

FePt纳米粒子 (NPs) 以其优异的超顺磁性和抗癌活性在生物医学领域引起了广泛的关注, 作为MRI/CT造影剂和纳米抗癌药物在肿瘤检测和治疗中具有巨大的应用潜力。在本研究中, 我们研究了FePt NPs对神经胶质瘤U251细胞增殖的抑制机制。不同浓度的FePt NPs与U251细胞共培养, 通过检测细胞内各种氧化还原酶的活性和几种凋亡相关蛋白的表达, 研究细胞内的氧化应激状态。细胞活性测定表明: 5 μg·ml-1 的FePt NPs处理24小时后, 对U251细胞增殖的最高抑制率约为10.5%±5.6%。在这种情况下, LDH释放显著增加。流式细胞术和荧光染色分析表明, FePt-NPs促进U251细胞凋亡和G0/G1期阻滞。生化分析表明: 不同浓度的FePt NPs处理后, U251细胞的氧化还原酶 (SOD, CAT和GPx) 活性显著降低, 同时细胞内脂质过氧化产物 (MDA) 水平升高。Western blotting分析进一步表明: FePt NPs上调促凋亡蛋白Bax的表达, 下调抗凋亡蛋白Bcl-2和抑癌蛋白p53的表达。这些结果证明FePt NPs通过诱导细胞内氧化应激抑制神经胶质瘤U251细胞的增殖。

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Acknowledgements

This study was financially supported by the Opening fund of Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica (No. BCMM201806) and the Basic Research Project of Wuhan Science and Technology Bureau (No. 2014060101010041).

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Authors and Affiliations

  1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomedical Materials and Engineering Centre, Wuhan University of Technology, Wuhan, 430070, China

    Yao-Min Li, Li-Hui Meng, Yan-Ru Hu, Tian-Tian Zhang, Zheng-Nan Su, Zhi-Yuan Ouyang, Wen-Chao Li & Qing-Zhi Wu

  2. National Engineering Research Centre for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Jiang-Ling Wan

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Li, YM., Meng, LH., Hu, YR. et al. Suppression mechanisms on proliferation of glioma U251 cells by FePt nanoparticles through intracellular oxidative stress. Rare Met. 41, 1202–1209 (2022). https://doi.org/10.1007/s12598-021-01885-z

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