Skip to main content
SpringerLink
Log in

Genotypes, haplotypes and diplotypes of IGF-II SNPs and their association with growth traits in largemouth bass (Micropterus salmoides)

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Insulin-like growth factor II (IGF-II) is involved in the regulation of somatic growth and metabolism in many fishes. IGF-II is an important candidate gene for growth traits in fishes and its polymorphisms were associated with the growth traits. The aim of this study is to screen single nucleotide polymorphisms (SNPs) of the largemouth bass (Micropterus salmoides) IGF-II gene and to analyze potential association between IGF-II gene polymorphisms and growth traits in largemouth bass. Four SNPs (C127T, T1012G, C1836T and C1861T) were detected and verified by DNA sequencing in the largemouth bass IGF-II gene. These SNPs were found to organize into seven haplotypes, which formed 13 diplotypes (haplotype pairs). Association analysis showed that four individual SNPs were not significantly associated with growth traits. Significant associations were, however, noted between diplotypes and growth traits (P < 0.05). The fish with H1H3 (CTCC/CGCC) and H1H5 (CTCC/TTTT) had greater body weight than those with H1H1 (CTCC/CTCC), H1H2 (CTCC/TGTT) and H4H4 (TGCT/TGCT/) did. Our data suggest a significant association between genetic variations in the largemouth bass IGF-II gene and growth traits. IGF-II SNPs could be used as potential genetic markers in future breeding programs of largemouth bass.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Greene MW, Chen TT (1997) Temporal expression pattern of insulin-like growth factor mRNA during embryonic development in a teleost, rainbow trout (Onchorynchus mykiss). Mol Mar Biol Biotechnol 6:144–151

    PubMed  CAS  Google Scholar 

  2. O’Dell SD, Day IN (1998) Insulin-like growth factor II (IGF-II). Int J Biochem Cell Biol 30:767–771

    Article  PubMed  Google Scholar 

  3. Florini JR, Magri KA, Ewton DZ, James PL, Grindstaff K, Rotwein PS (1991) “Spontaneous” differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. J Biol Chem 266:15917–15923

    PubMed  CAS  Google Scholar 

  4. Gomes MV, Soares MR, Pasqualim-Neto A, Marcondes CR, Lôbo RB, Ramos ES (2005) Association between birth weight, body mass index and IGF2/ApaI polymorphism. Growth Horm IGF Res 15:360–362

    Article  PubMed  CAS  Google Scholar 

  5. Zhang W, Maniatis N, Rodriguez S, Miller GJ, Day IN, Gaunt TR, Collins A, Morton NE (2006) Refined association mapping for a quantitative trait: weight in the H19-IGF2-INS-TH region. Ann Hum Genet 70:848–856

    Article  PubMed  CAS  Google Scholar 

  6. Van Laere AS, Nguyen M, Braunschweig M, Nezer C, Collette C, Moreau L, Archibald AL, Haley CS, Buys N, Tally M, Andersson G, Georges M, Andersson L (2003) A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425:832–836

    Article  PubMed  Google Scholar 

  7. Goodall JJ, Schmutz SM (2007) IGF2 gene characterization and association with rib eye area in beef cattle. Anim Genet 38:154–161

    Article  PubMed  CAS  Google Scholar 

  8. Ron M, Weller JI (2007) From QTL to QTN identification in livestock “winning by points rather than knock-out”. Anim Genet 38:429–439

    Article  PubMed  CAS  Google Scholar 

  9. Duguay SJ, Lai-Zhang J, Steiner DF, Funkenstein B, Chan SJ (1996) Developmental and tissue-regulated expression of IGF-I and IGF-II mRNAs in Sparus aurata. J Mol Endocrinol 16:123–132

    Article  PubMed  CAS  Google Scholar 

  10. Collet C, Candy J, Richardson N, Sara V (1997) Organization, sequence, and expression of the gene encoding IGF-II from barramundi (Teleosteii; Lates calcarifer). Biochem Genet 35:211–224

    Article  PubMed  CAS  Google Scholar 

  11. Radaelli G, Patruno M, Maccatrozzo L, Funkenstein B (2003) Expression and cellular localization of insulin-like growth factor-II protein and mRNA in Sparus aurata during development. J Endocrinol 178:285–299

    Article  PubMed  CAS  Google Scholar 

  12. Codina M, García de la serrana D, Sánchez-Gurmaches J, Montserrat N, Chistyakova O, Navarro I, Gutiérrez J (2008) Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways. Gen Comp Endocrinol 157:116–124

    Article  PubMed  CAS  Google Scholar 

  13. Vong QP, Chan KM, Cheng CH (2003) Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by real-time PCR: differential regulation of expression by GH. J Endocrinol 178:513–521

    Article  PubMed  CAS  Google Scholar 

  14. Carnevali O, Cardinali M, Maradonna F, Parisi M, Olivotto I, Polzonetti-Magni AM, Mosconi G, Funkenstein B (2005) Hormonal regulation of hepatic IGF-I and IGF-II gene expression in the marine teleost Sparus aurata. Mol Reprod Dev 71:12–18

    Article  PubMed  CAS  Google Scholar 

  15. Peterson BC, Waldbieser GC, Bilodeau L (2004) IGF-I and IGF-II mRNA expression in slow and fast growing families of USDA103 channel catfish (Ictalurus punctatus). Comp Biochem Physiol A Mol Integr Physiol 139:317–323

    Article  PubMed  Google Scholar 

  16. Wang CM, Lo LC, Feng F, Zhu ZY, Yue GH (2008) Identification and verification of QTL associated with growth traits in two genetic backgrounds of barramundi (Lates calcarifer). Anim Genet 39:34–39

    Article  PubMed  Google Scholar 

  17. Yu JH, Chen XF, Li JL, Tang YK, Li HX, Xu P, Dong ZJ (2010) Isolation of IGF2 and association of IGF2 polymorphism with growth trait in genetically improved farmed tilapias, Oreochromis niloticus L. Aquac Res 11:1–8

    CAS  Google Scholar 

  18. Bai JJ, Lutz-Carrillo DJ, Quan YC, Liang SX (2008) Taxonomic status and genetic diversity of cultured largemouth bass Micropterus salmoides in China. Aquaculture 278:27–30

    Article  CAS  Google Scholar 

  19. Li XH, Bai JJ, Ye X, Hu YC, Li SJ, Yu LY (2009) Polymorphisms in the 5′ flanking region of the insulin-like growth factor I gene are associated with growth traits in largemouth bass Micropterus salmoides. Fisheries Sci 75:351–358

    Article  CAS  Google Scholar 

  20. Chen JY, Tsai HL, Chang CY, Wang JI, Shen SC, Wu JL (1998) Isolation and characterization of tilapia (Oreochromis mossambicus) insulin-like growth factors gene and proximal promoter region. DNA Cell Biol 17:359–376

    Article  PubMed  CAS  Google Scholar 

  21. Terova G, Rimoldi S, Chini V, Gornati R, Bernardini G, Saroglia M (2007) Cloning and expression analysis of insulin-like growth factor I and II in liver and muscle of sea bass (Dicentrarchus labrax, L) during long-term fasting and refeeding. J Fish Biol 70(Suppl B):219–233

    Article  CAS  Google Scholar 

  22. Ponce M, Infante C, Funes V, Manchado M (2008) Molecular characterization and gene expression analysis of insulin-like growth factors I and II in the redbanded seabream, Pagrus auriga: transcriptional regulation by growth hormone. Comp Biochem Physiol B Biochem Mol Biol 150:418–426

    Article  PubMed  Google Scholar 

  23. Wingender E, Chen X, Fricke E, Geffers R, Hehl R, Liebich I, Krull M, Matys V, Michael H, Ohnhäuser R, Prüss M, Schacherer F, Thiele S, Urbach S (2001) The TRANSFAC system on gene expression regulation. Nucleic Acids Res 29:281–283

    Article  PubMed  CAS  Google Scholar 

  24. Tse MC, Chan KM, Cheng CH (2008) Cloning, characterization and promoter analysis of the common carp IGF-II gene. Gene 412:26–38

    Article  PubMed  CAS  Google Scholar 

  25. Palamarchuk AY, Holthuizen PE, Müller WE, Sussenbach JS, Kavsan VM (1997) Organization and expression of the chum salmon insulin-like growth factor II gene. FEBS Lett 1997:344–348

    Article  Google Scholar 

  26. Chen JY, Chang CY, Chen JC, Shen SC, Wu JL (1997) Production of biologically active recombinant tilapia insulin-like growth factor II polypeptides in E. coli cells and characterization of the genomic structure of the coding region. DNA Cell Biol 16:883–892

    Article  PubMed  CAS  Google Scholar 

  27. Shamblott MJ, Leung S, Greene MW, Chen TT (1998) Characterization of a teleost insulin-like growth factor II (IGF-II) gene: evidence for promoter CCAAT/enhancer-binding protein (C/EBP) sites, and the presence of hepatic C/EBP. Mol Mar Biol Biotechnol 7:181–190

    PubMed  CAS  Google Scholar 

  28. Li XH, Bai JJ, Ye X, Hu YC (2009) Polymorphisms of intron 1, 3, and 4 of insulin-like growth factor I gene in largemouth bass. J Shanghai Ocean University 18:8–13 (In Chinese, with English abstract)

    Google Scholar 

  29. Yu LY, Bai JJ, Ye X, Li SJ, Li XH (2009) Genome structure of the largemouth bass MyoD gene and its single nucleotide polymorphisms screening. J Fisheries China 33:1–7 (In Chinese, with English abstract)

    CAS  Google Scholar 

  30. Drysdale CM, McGraw DW, Stack CB, Stephens JC, Judson RS, Nandabalan K, Arnold K, Ruano G, Liggett SB (2000) Complex promoter and coding region beta 2-adrenergic receptor haplotypes alter receptor expression and predict in vivo responsiveness. Proc Natl Acad Sci USA 97:10483–10488

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Gao Feng Ying, Hong Mei Song, Xiao Yan He and Jia Jia Fan for their kind help in collecting blood samples and measuring body sizes of fish. This work was supported by grants from the National Natural Science Foundation of China (Grant No. 30901102), the Natural Science Foundation of Guangdong, China (Grant No. 9151038001000005), Science-Technology Supporting Project of the National ‘Eleventh Five-Year-Plan’ of China (Grant No. 2006BAD01A1209) and The Special Fund for Public Welfare Industry (Agriculture) Research (200903045).

Author information

Authors and Affiliations

  1. Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture of the People’s Republic of China, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, Guangdong, People’s Republic of China

    Xiaohui Li, Junjie Bai, Yinchang Hu, Xing Ye, Shengjie Li & Lingyun Yu

  2. College of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing, 210003, Jiangsu, China

    Xiaohui Li

Authors
  1. Xiaohui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Junjie Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yinchang Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xing Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shengjie Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lingyun Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Junjie Bai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, X., Bai, J., Hu, Y. et al. Genotypes, haplotypes and diplotypes of IGF-II SNPs and their association with growth traits in largemouth bass (Micropterus salmoides). Mol Biol Rep 39, 4359–4365 (2012). https://doi.org/10.1007/s11033-011-1223-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-011-1223-2

Keywords

Search

Navigation