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Proteomic Analysis of Salt Stress Responses in Rice Shoot

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Abstract

To gain a better understanding of the mechanism of rice (Oryza sativa L.) in response to salt stress, we performed a proteomics analysis of rice in response to 250 mM NaCl treatment using shoots of 3-day-old nascent seedlings. The changes of protein patterns were monitored with two-dimensional gel electrophoresis. Of 57 protein spots showing changes in abundance in response to salt stress, 52 were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The identified proteins were classified into eight functional categories. Several novel salt stress-responsive proteins, including protein synthesis inhibitor I, photosystem II stability/assembly factor HCF136, trigger factor-like protein and cycloartenol-C24-methyltransferase are upregulated upon salt stress. In order to figure out the different and similar molecular mechanism among salt and other stresses, regulation of some salt responsive proteins under other abiotic stress (cold and dehydration) and abscisic acid application was also analyzed. The possible molecular mechanism of rice seedlings in response to salinity and other stresses were discussed.

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Acknowledgments

This work was supported by the Genetically Modified Organisms Breeding Major Projects (2009ZX08009-074B).

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Author notes

  1. Xiao-Juan Li

    Present address: College of Life Sciences, Agriculture University of Hebei, Baoding, 071001, People’s Republic of China

Authors and Affiliations

  1. Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People’s Republic of China

    Xiao-Juan Li, Ming-Feng Yang, Yu Liang & Shi-Hua Shen

  2. College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, People’s Republic of China

    Yi Zhu

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  1. Xiao-Juan Li
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Correspondence to Shi-Hua Shen.

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Figure S1

Comparison of the proteome patterns of the 3-day-old rice seedlings after treatments of 48 h: A, B, and C represent the maps of ABA, drought, and cold treatment, respectively. Differentially regulated protein spots in every treatment are circled and indicated by arrows (DOC 13,091 kb)

Table S1

Regulation of salt responsive proteins under different treatments (DOC 96 kb)

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Li, XJ., Yang, MF., Zhu, Y. et al. Proteomic Analysis of Salt Stress Responses in Rice Shoot. J. Plant Biol. 54, 384–395 (2011). https://doi.org/10.1007/s12374-011-9173-8

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