Abstract
Dextranase from Chaetomium gracile is generally considered safe for use in the sugarcane industry. Herein, a truncated and codon-optimised α-dextranase gene from C. gracile was successfully cloned and expressed in Saccharomyces cerevisiae for the first time. The optimum conditions of fermentation was achieved when the maximum dextranase activity reached to 58.45 U/mL after 48 h in shake flasks. The optimal pH and temperature were 5.5 and 60 °C, respectively. The recombinant dextranase remained stable between pH 4 and 6 and temperature between 55 and 60 °C. The findings in the present study could facilitate large-scale production of food-grade recombinant dextranase for use in the sugar industry.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (31460026), the National Natural Science Foundation of Guangxi Province (2018GXNSFAA050126), the Bosch Young Teachers Innovation Training Project (BRP180215) and the Fundamental Research Fund of Guangxi University (XJZ140293).
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Liu, J., Sun, Q., Yin, H. et al. Optimal Fermentation of Saccharomyces cerevisiae Expressing a Dextranase from Chaetomium gracile. Sugar Tech 22, 171–178 (2020). https://doi.org/10.1007/s12355-019-00746-5
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DOI: https://doi.org/10.1007/s12355-019-00746-5