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Changes in Biological Nitrogen Fixation and Natural-Abundance N Isotopes of Sugarcane Under Molybdenum Fertilization

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Abstract

Molybdenum (Mo) can stimulate nitrogenase activity (NTA), increase biological nitrogen (N) fixation (BNF) and reduce natural-abundance N isotopes (δ15N). Our hypothesis is that this stimulus depends on genotype and N availability for sugarcane. The objective of this study was to evaluate the effect of the Mo fertilization on BNF, NTA, δ15N and the accumulation of N in different genotypes (RB867515 and RB92579) of sugarcane fertilized with N. The cultivars were subjected to two rates of N fertilization (without N fertilization and 60 kg ha−1) and two rates of Mo fertilization (without Mo fertilization and 200 g ha−1). NTA, δ15N and BNF were evaluated throughout the cycle of the sugarcane. Both leaf and root NTA was maximal at approximately 100 DAP. Mo fertilization stimulated NTA in RB867515 but reduced in RB92579. The bacteria associated with the sugarcane fixed atmospheric-N2 and the Mo fertilization stimulated this fixation, especially without N fertilization. Mo fertilization promoted greater N accumulation and should be encouraged for fertility management, especially in soils with low N content or as an alternative for reducing the use of N fertilizers in sugarcane.

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Acknowledgements

The authors would like to thank the National Council for Scientific and Technological Development (CNPq) and the Coordination of Higher Level Personnel (CAPES) for granting the scholarships, and the scholars and volunteers for their help in conducting the experiment and colleting the data. This study was supported by CNPq Project No. 471575/2009-7 entitled “Evaluation of the availability of molybdenum to sugarcane and its contribution to nitrogen fertilization and nitrogen fixation”.

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Authors and Affiliations

  1. Instituto Federal de Educação, Ciência e Tecnologia, Vitória de Santo Antão, Brazil

    Renato Lemos dos Santos

  2. Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, Brazil

    Fernando José Freire, Emídio Cantídio Almeida de Oliveira, Maria Betânia Galvão dos Santos Freire, Patrícia da Costa Bezerra & Ruthanna Izabelle de Oliveira

  3. Laboratório de Isótopos Estáveis, Centro de Energia Nuclear na Agricultura, Piracicaba, Brazil

    Paulo Cesar Ocheuze Trivelin

  4. Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Brazil

    Monalisa Barbosa da Costa Santos

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  1. Renato Lemos dos Santos
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  2. Fernando José Freire
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  3. Emídio Cantídio Almeida de Oliveira
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Correspondence to Fernando José Freire.

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dos Santos, R.L., Freire, F.J., de Oliveira, E.C.A. et al. Changes in Biological Nitrogen Fixation and Natural-Abundance N Isotopes of Sugarcane Under Molybdenum Fertilization. Sugar Tech 21, 925–935 (2019). https://doi.org/10.1007/s12355-019-00717-w

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