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Biocontrol Strategies to Manage Fungal Diseases in Sugarcane

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Abstract

Red rot, wilt, sett rot and seedling rot are the major fungal diseases for which biological control could be used as effective tool as a component of integrated disease management. Efficient fungal and bacterial antagonists have been identified against them, and their efficacy has been proved under in vitro and in vivo conditions. For red rot, fungal bioagents, viz. Chaetomium, Trichoderma, and bacterial antagonists individually and in combination of bacterial antagonists and fungicide were found to be effective in protecting the crop. Capability of bacterial antagonists in inducing resistance through induced systemic resistance against red rot was established. Delivery of the antagonists through sett treatment was standardized for field application. Further, pressmud formulation of Trichoderma was found effective against wilt under field conditions. Similarly, use of Trichoderma formulation was highly effective against seedling rot caused by Pythium spp. and it is used in seedling trays to manage the disease. Attempts were made to isolate and characterize antifungal genes/proteins capable of reducing the pathogenic potential of Colletotrichum falcatum, the red rot pathogen, and have yielded desirable results. Recently, molecular approaches have been applied to understand antagonistic mechanism between C. falcatum and Trichoderma harzianum. In vitro and in vivo studies during tritrophic interactions among the antagonist, the host plant and the pathogen revealed key pathogenicity genes/proteins in C. falcatum targeted by T. harzianum to reduce pathogenicity. Through interactive proteomics, many candidate antifungal proteins were identified from T. harzianum. Recent studies on biocontrol of sugarcane diseases have revealed scope for its application in the field to manage these diseases and T. harzianum as a bridge species to identify pathogenicity genes of red rot pathogen and identifying candidate antifungal genes.

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References

  • Agnihotri, V.P., and G.P. Rao. 2002. A century status of sugarcane wilt in India. In Sugarcane Crop Management, ed. S.B. Singh, G.P. Rao, and S. Eswaramoorthy, 145–160. Houston: SciTech Publishing LLC.

    Google Scholar 

  • Bae, H., R.C. Sicher, M.S. Kim, S.H. Kim, M.D. Strem, R.L. Melnick, and B.A. Bailey. 2009. The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao. Journal of Experimental Botany 60: 3279–3295.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Bailey, B.A., H. Bae, M.D. Strem, D.P. Roberts, S.E. Thomas, J. Crozier, G.J. Samuels, I.Y. Choi, and K.A. Holmes. 2006. Fungal and plant gene expression during the colonization of cacao seedlings by endophytic isolates of four Trichoderma species. Planta 224: 1449–1464.

    Article  PubMed  CAS  Google Scholar 

  • Elamathi, E. 2017. Molecular analysis on antagonistic suppression of Colletotrichum falcatum causing red rot in sugarcane. Ph.D thesis, Bharathiar University, Coimbatore.

  • Elamathi, E., P. Malathi, R. Viswanathan, and A. Ramesh Sundar. 2016. Potential of Paenibacillus and Trichoderma isolates against Colletotrichum falcatum causing red rot disease in sugarcane. Journal of Sugarcane Research 6 (2): 100–111.

    Google Scholar 

  • Elamathi, E., P. Malathi, R. Viswanathan, and A. Ramesh Sundar. 2017. Molecular analysis of the suppression of Colletotrichum falcatum by Trichoderma harzianum in sugarcane. Journal of Plant Pathology 99: 211–218.

    Google Scholar 

  • Elamathi, E., P. Malathi, R. Viswanathan, and A. Ramesh Sundar. 2018. Expression analysis on mycoparasitism related genes during antagonism of Trichoderma with Colletotrichum falcatum causing red rot in sugarcane. Journal of Plant Biochemistry and Biotechnology. https://doi.org/10.1007/s13562-018-0444-z.

    Article  Google Scholar 

  • Gorlach, J., S. Volrath, G. Knauf-Beiter, G. Hengy, U. Beckhove, K. Kogel, M. Oostendorp, T. Staub, E. Ward, H. Kessmann, and J. Ryals. 1996. Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8: 629–643.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Goswami, D., S.N. Borah, J. Lahkar, P.J. Handique, and S. Deka. 2015. Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum. Journal of Basic Microbiology 55: 1–10.

    Article  CAS  Google Scholar 

  • Goswami, D., P.J. Handique, and S. Deka. 2014. Rhamnolipid biosurfactant against Fusarium sacchari-the causal organism of pokkah boeng disease of sugarcane. Journal of Basic Microbiology 54: 548–557.

    Article  PubMed  CAS  Google Scholar 

  • Gururaja, N. 1992. Studies on the possibility of control of red rot disease of sugarcane through chemical, physical and biological means. M.Sc (Agri.) Thesis, Tamil Nadu Agricultural University, Coimbatore, p 124.

  • Hassan, M.N., S. Afghan, and F.Y. Hafeez. 2012. Biological suppression of sugarcane red rot by Bacillus spp. under field conditions. Journal of Plant Pathology 94: 325–329.

    Google Scholar 

  • Hassan, M.N., S.Z. Namood-e-Sahar, S.Afghan Shah, and F.Y. Hafeez. 2015. Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake. BioControl 60: 691–702.

    Article  CAS  Google Scholar 

  • Joshi, D., P. Singh, S.K. Holkar, and S. Kumar. 2018. Trichoderma-mediated suppression of red rot of sugarcane under field conditions in subtropical India. Sugar Tech 8: 8–9. https://doi.org/10.1007/s12355-018-0624-0.

    Article  CAS  Google Scholar 

  • Joshi, D., P. Singh, A.K. Singh, R.J. Lal, and N. Tripathi. 2016. Antifungal potential of metabolites from Trichoderma sp. against Colletotrichum falcatum Went causing red rot of sugarcane. Sugar Tech 18: 529–536.

    Article  CAS  Google Scholar 

  • Malathi, P., P. Padmanaban, R. Viswanathan, and D. Mohanraj. 2008. Interaction between Colletotrichum falcatum pathotypes and biocontrol agents. Archives of Phytopathology and Plant Protection 41: 311–317.

    Article  Google Scholar 

  • Malathi, P., A. Ramesh Sundar, P. Padmanaban, and R. Viswanathan. 2001. Efficacy of biocontrol agents against sugarcane seedling rot. Sugarcane Breeding Institute Newsletter 20 (4): 3.

    Google Scholar 

  • Malathi, P., R. Viswanathan, P. Padmanaban, D. Mohanraj, and A. Ramesh Sundar. 2002a. Compatibility of biocontrol agents with fungicides against red rot disease of sugarcane. Sugar Tech 4 (3&4): 131–136.

    Article  Google Scholar 

  • Malathi, P., R. Viswanathan, P. Padmanaban, D. Mohanraj, and A. Ramesh Sundar. 2002b. Microbial detoxification of Colletotrichum falcatum toxin. Current Science 83: 745–749.

    CAS  Google Scholar 

  • Malathi, P., R. Viswanathan, and P. Padmanaban. 2012. Identification of antifungal proteins from fungal and bacterial antagonists against Colletotrichum falcatum causing sugarcane red rot. Journal of Biological Control 26: 49–54.

    Google Scholar 

  • Malathi, P., R. Viswanathan, and A. Ramesh Sundar. 2017. Mechanized means of sett treatment: An effective way of delivering fungicides for the management of red rot in sugarcane. Sugar Tech 19: 176–182.

    Article  CAS  Google Scholar 

  • Mohanraj, D., P. Padmanaban, and R. Viswanathan. 2002. Biological control of major diseases of sugarcane. In Biological Control of Major Crop Plant Diseases, ed. S.S. Gnanamanickam, 161–178. New York: Marcel Dekker Inc.

    Google Scholar 

  • Nallathambi, P., D. Mohanraj, and P. Padmanaban. 1997. Biocontrol of red rot caused by Colletotrichum falcatum Went in sugarcane. In Proceeding of International Symposium Integrated Disease Management for Sustainable Agriculture, New Delhi. pp 192.

  • Prabhukarthikeyan, S.R., R. Manikandan, D. Durgadevi, U. Keerthana, S. Harish, G. Karthikeyan, and T. Raguchander. 2017. Bio-suppression of turmeric rhizome rot disease and understanding the molecular basis of tripartite interaction among Curcuma longa, Pythium aphanidermatum and Pseudomonas fluorescens. Biological Control 111: 23–31.

    Article  CAS  Google Scholar 

  • Ramamoorthy, V., R. Viswanathan, T. Raghuchander, V. Prakasam, and R. Samiyappan. 2001. Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protection 20: 1–11.

    Article  CAS  Google Scholar 

  • Ramesh Sundar, A., E. Leonard Barnabas, P. Malathi, and R. Viswanathan. 2012. A mini-review on smut disease of sugarcane caused by Sporisorium scitamineum. In Botany, ed. J.K. Mworia, 107–128. Rijeka: InTech.

    Google Scholar 

  • Senthil, N., T. Raguchander, R. Viswanathan, and R. Samiyappan. 2003. Talc formulated fluorescent pseudomonads for sugarcane red rot suppression and enhanced yield under field conditions. Sugar Tech 5 (1&2): 37–43.

    Article  CAS  Google Scholar 

  • Singh, N. 1994. Trichoderma harzianum and Chaetomium sp. as potential biocontrol fungi in management of red rot disease of sugarcane. Journal of Biological Control 8: 65–67.

    Google Scholar 

  • Singh, N. 1999. Biological control of red rot. Annual Report. Sugarcane Breeding Institute, 1998–1999, pp 56.

  • Singh, N. 2002. Management of red rot disease of sugarcane through biotherapy. In Sugarcane Crop Management, ed. S.B. Singh, G.P. Rao, and S. Eswaramoorthy, 351–363. Houston, TX: SCI TECH Publishing LLC.

    Google Scholar 

  • Singh, V., and B.B. Joshi. 2007. Mass multiplication of Trichoderma harzianum on sugarcane press mud. Indian Phytopathology 60: 530–531.

    Google Scholar 

  • Singh, V., B.B. Joshi, S.K. Awasthi, and S.N. Srivastava. 2008. Eco-friendly management of red rot disease of sugarcane with Trichoderma strains. Sugar Tech 10: 158–161.

    Article  Google Scholar 

  • Sticher, L., B. Mauch-Mani, and J.P. Metraux. 1997. Systemic acquired resistance. Annual Review of Phytopathology 35: 235–270.

    Article  PubMed  CAS  Google Scholar 

  • Van Loon, L.C., P.A.H.M. Bakker, and C.M.J. Pieterse. 1998. Systemic resistance induced by rhizosphere bacteria. Annual Review of Phytopathology 36: 453–483.

    Article  PubMed  Google Scholar 

  • Vieira, P.M., A.S.G. Coelho, A.S. Steindorff, S.J.L. de Siqueira, R. do Nascimento Silva, and C.J. Ulhoa. 2013. Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application. BMC Genomics 14 (1): 177.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Viswanathan, R. 2006. Achievements in biological control of diseases of sugarcane with antagonistic organisms at Sugarcane Breeding Institute, Coimbatore. In Current status of Biological Control of Plant Diseases Using Antagonistic Organisms in India, ed. B. Ramanujam and R.J. Rabindra, 304–309. Bangalore: Project Directorate of Biological Control.

    Google Scholar 

  • Viswanathan, R. 2010. Plant Disease: Red Rot of Sugarcane. New Delhi: Anmol Publishers.

    Google Scholar 

  • Viswanathan, R. 2012. Sugarcane Diseases and Their Management. Coimbatore: Sugarcane Breeding Institute.

    Google Scholar 

  • Viswanathan, R. 2013. Status of sugarcane wilt: One 100 years after its occurrence in India. Journal of Sugarcane Research 3 (2): 86–106.

    Google Scholar 

  • Viswanathan, R., P. Malathi, and R. Naik. 2016. A sett treatment device for fungal disease management and healthy nursery programme in sugarcane. Proceedings of Annual Convention of Sugar Technologists of India 77: 236–253.

    Google Scholar 

  • Viswanathan, R.P., C.N. Malathi, R. Naik Prasanth, and S.J.K. Annamalai. 2017a. Sett way of growing sugarcane for fungal treatment. ICAR News 23 (2): 1–3.

    Google Scholar 

  • Viswanathan, R., P. Malathi, A. Ramesh Sundar, K. Kaverinathan, M.L. Chhabra, B. Parameswari, and R. Jothi. 2017b. Diversity of Colletotrichum falcatum population in India: Comparative virulence at two different agro-climatic regions. International Sugar Journal 119: 966–977.

    Google Scholar 

  • Viswanathan, R.P., C.N. Malathi, A. Prasanth, and M.Scindiya Annadurai. 2014. Sudden occurrence of wilt and pokkah boeng in sugarcane and status of resistance in the parental clones in national hybridization garden to these diseases. Journal of Sugarcane Research 4 (1): 62–81.

    Google Scholar 

  • Viswanathan, R., P. Malathi, A. Ramesh Sundar, M. Poongothai, and N. Singh. 2006a. Current status of sugarcane wilt in India. Sugar Cane International 24 (4): 2–7.

    Google Scholar 

  • Viswanathan, R., R. Nandakumar, and R. Samiyappan. 2003a. Involvement of pathogenesis-related proteins in Pseudomonas spp. induced systemic resistance against Colletotrichum falcatum in sugarcane. Journal of Plant Diseases and Protection 110: 524–537.

    CAS  Google Scholar 

  • Viswanathan, R., and P. Padmanaban. 2008. Hand Book on Sugarcane Diseases and Their Management. Coimbatore: Sugarcane Breeding Institute.

    Google Scholar 

  • Viswanathan, R., M. Poongothai, P. Malathi, and A. Ramesh Sundar. 2012. Sugarcane Wilt: New insights into the pathogen identity, variability and pathogenicity. Functional Plant Science and Biotechnology 6: 30–39.

    Google Scholar 

  • Viswanathan, R., S.M. Premkumari, A. Ramesh Sundar, and T. Kathiresan. 2006b. Cloning partial endochitinase cDNA of Trichoderma harzianum antagonistic to Colletotrichum falcatum causing red rot of sugarcane. Current Science 91: 951–956.

    CAS  Google Scholar 

  • Viswanathan, R., R. Rajitha, A. Ramesh Sundar, and V. Ramamoorthy. 2003b. Isolation and characterization of endophytic bacterial strains from sugarcane stalks and their in vitro antagonism against the red rot pathogen. Sugar Tech 5 (1–2): 25–29.

    Article  CAS  Google Scholar 

  • Viswanathan, R., A. Ramesh Sundar, and S.M. Premkumari. 2003c. Mycolytic enzymes of extracellular enzymes of antagonistic microbes to Colletotrichum falcatum, red rot pathogen of sugarcane. World Journal of Microbiology & Biotechnology 19: 953–959.

    Article  CAS  Google Scholar 

  • Viswanathan, R., and G.P. Rao. 2011. Disease scenario and management of major sugarcane diseases in India. Sugar Tech 13 (4): 336–353.

    Article  CAS  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 1999a. Induction of systemic resistance by plant growth promoting rhizobacteria against red rot disease in sugarcane. Sugar Tech 1 (3): 67–76.

    Article  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 1999b. Management of damping off disease in sugarcane using plant growth promoting rhizobacteria. Madras Agricultural Journal 86: 647–649.

    Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2000. Efficacy of Pseudomonas spp. strains against soil borne and sett borne inoculum of Colletotrichum falcatum causing red rot disease of sugarcane. Sugar Tech 2 (3): 26–29.

    Article  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2001a. Antagonism of different Pseudomonas strains against red rot pathogen, Colletotrichum falcatum. Indian Journal of Sugarcane Technology 16 (1): 44–49.

    Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2001b. Antifungal activity of chitinases produced by some fluorescent pseudomonads against Colletotrichum falcatum Went causing red rot disease in sugarcane. Microbiological Research 155: 309–314.

    Article  PubMed  CAS  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2001c. Role of chitinases in Pseudomonas spp. induced systemic resistance against Colletotrichum falcatum in sugarcane. Indian Phytopathology 54: 418–423.

    CAS  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2002a. Induced systemic resistance by fluorescent pseudomonads against red rot disease of sugarcane caused by Colletotrichum falcatum. Crop Protection 21: 1–10.

    Article  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2002b. Role of oxidative enzymes in the plant growth promoting rhizobacteria (PGPR) mediated induced systemic resistance against Colletotrichum falcatum in sugarcane. Journal of Plant Diseases and Protection 109: 88–100.

    CAS  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2004. Secondary metabolites production by Pseudomonas spp. strains antagonistic to Colletotrichum falcatum causing red rot disease in sugarcane. Acta Phytopathologica Entomologica et Hungarica 39 (1–3): 28–37.

    Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2006. Pseudomonas spp. colonization in sugarcane rhizosphere reduces titre of Colletotrichum falcatum Went-causing red rot disease of sugarcane. Archives of Phytopathology and Plant Protection 39: 39–44.

    Article  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2007. Siderophores and iron nutrition on the pseudomonas mediated antagonism against Colletotrichum falcatum in sugarcane. Sugar Tech 9 (1): 57–60.

    Article  CAS  Google Scholar 

  • Viswanathan, R., and R. Samiyappan. 2008. Bio-formulation of fluorescent Pseudomonas spp. induces systemic resistance against red rot disease and enhances commercial sugar yield in sugarcane. Archives of Phytopathology and Plant Protection 41: 377–388.

    Article  CAS  Google Scholar 

  • Viswanathan, R., R. Samiyappan, P. Nallathambi, and V. Ganesan. 2000. Plant growth promotion by native fluorescent Pseudomonas in sugarcane crop. Proceedings of Annual Convention of Sugar Technologists Association of India 62: 16–35.

    Google Scholar 

  • Yadav, R.L., V. Singh, S.N. Srivastav, R.J. Lal, S. Srivastav, S.K. Awasthi, and B.B. Joshi. 2008. Use of Trichoderma harzianum for the control of red rot disease of sugarcane. Sugar Cane International 26 (4): 26–31.

    Google Scholar 

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Acknowledgements

The authors are grateful to the funding agencies, ICAR and Sugar Development Fund for supporting the research activities. Encouragement from the Directors of the Institute and support from the sugar factories are gratefully acknowledged.

Funding

This study was supported by different projects from AP Cess Fund and network projects of ICAR and Sugar Development Fund and ICAR-SBI.

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  1. Division of Crop Protection, Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, 641007, India

    R. Viswanathan & P. Malathi

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Viswanathan, R., Malathi, P. Biocontrol Strategies to Manage Fungal Diseases in Sugarcane. Sugar Tech 21, 202–212 (2019). https://doi.org/10.1007/s12355-018-0690-3

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