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Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake

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Abstract

Red rot disease affects sugarcane production adversely throughout the world. Intensive cropping and uninterrupted use of chemicals results in evolution of new pathotypes which can infect the resistant cultivars. Bacillus sp. inhabiting plant rhizosphere can protect the plants from multiple pathogens. In the present study, two antagonistic strains Bacillus subtilis NH-100 and Bacillus sp. NH-217 were able to maintain their population at 9.0 log CFU g−1 in sugarcane filter cake until nine months. These strains were compatibile to the indigenous bacteria of sugarcane filter cake which maintained their population at 7.8–8.0 log CFU g−1. The developed bioformulation induced the activity of various defense-related enzymes (0.6–6.9 change in absorbance min−1 g−1 of tissue), suppressed the red rot disease and enhanced crop yield under field conditions. This formulation could be used as potential biopesticide to control red rot disease of sugarcane.

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Acknowledgments

This work was supported by the Pakistan Science Foundation under Research Grant #PSF/NSLP/CIIT-C-79. We thank Dr. G. Defago (Plant pathology, Institute of Integrative Biology Zürich, Switzerland) for providing strain P. fluorescens CHA0 to be used as positive controls in this study. We are also thankful to Dr. Muhammad Umer for improving the English in the manuscript, Dr. Syed Tahir Abbas Shah and Dr. Mustafa Nawaz Shafqat, department of biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan for helping in statistical analysis of the data.

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Authors and Affiliations

  1. COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan

    Muhammad Nadeem Hassan,  Namood-e-Sahar & Fauzia Yusuf Hafeez

  2. Shakarganj Sugar Research Institute (SSRI), Jhang, Pakistan

    Syed Zia-Ul-Husnain Shah & Shahid Afghan

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  1. Muhammad Nadeem Hassan
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  2. Namood-e-Sahar
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  3. Syed Zia-Ul-Husnain Shah
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  4. Shahid Afghan
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  5. Fauzia Yusuf Hafeez
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Correspondence to Muhammad Nadeem Hassan.

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Handling Editor: Fouad Daayf.

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Hassan, M.N., Namood-e-Sahar, Zia-Ul-Husnain Shah, S. et al. Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake. BioControl 60, 691–702 (2015). https://doi.org/10.1007/s10526-015-9673-4

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  • DOI: https://doi.org/10.1007/s10526-015-9673-4

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