Abstract
Non-invasive stimulation of the human cerebellum, such as by transcranial magnetic stimulation (TMS), is increasingly used to investigate cerebellar function and identify potential treatment for cerebellar dysfunction. However, the effects of TMS on cerebellar neurons remain poorly defined. We applied low-intensity repetitive TMS (LI-rTMS) to the mouse cerebellum in vivo and in vitro and examined the cellular and molecular sequelae. In normal C57/Bl6 mice, 4 weeks of LI-rTMS using a complex biomimetic high-frequency stimulation (BHFS) alters Purkinje cell (PC) dendritic and spine morphology; the effects persist 4 weeks after the end of stimulation. We then evaluated whether LI-rTMS could induce climbing fibre (CF) reinnervation to denervated PCs. After unilateral pedunculotomy in adult mice and 2 weeks sham or BHFS stimulation, VGLUT2 immunohistochemistry was used to quantify CF reinnervation. In contrast to sham, LI-rTMS induced CF reinnervation to the denervated hemicerebellum. To examine potential mechanisms underlying the LI-rTMS effect, we verified that BHFS could induce CF reinnervation using our in vitro olivocerebellar explants in which denervated cerebellar tissue is co-cultured adjacent to intact cerebella and treated with brain-derived neurotrophic factor (BDNF) (as a positive control), sham or LI-rTMS for 2 weeks. Compared with sham, BDNF and BHFS LI-rTMS significantly increased CF reinnervation, without additive effect. To identify potential underlying mechanisms, we examined intracellular calcium flux during the 10-min stimulation. Complex high-frequency stimulation increased intracellular calcium by release from intracellular stores. Thus, even at low intensity, rTMS modifies PC structure and induces CF reinnervation.
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Acknowledgments
We wish to thank Tom Dufor, Anaëlle Dumas, Jennifer Fransson and Alix Noel de la Font Reaulx for technical assistance. The study was supported in part by the Institut de la Recherche sur la Moelle épinière et l’Encéphale in France and the Neurotrauma Research Program Western Australia.
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There are no current or potential conflicts of interest for any of the authors.
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N Morellini and S Grehl contributed equally to this work.
AM Lohof and RM Sherrard are co-senior authors.
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Morellini, N., Grehl, S., Tang, A. et al. What Does Low-Intensity rTMS Do to the Cerebellum?. Cerebellum 14, 23–26 (2015). https://doi.org/10.1007/s12311-014-0617-9
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DOI: https://doi.org/10.1007/s12311-014-0617-9