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Physiological and metabolic changes in two Himalayan medicinal herbs under drought, heat and combined stresses

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Abstract

Valeriana jatamansi Jones and Hedychium spicatum Ham-ex-Smith are important medicinal herbs of the Himalayan region, which are highly demanded by pharmaceutical industries. Climatic variability especially increasing temperature and water deficit affects the growth and productivity of these species. In addition, increased temperature and water deficit may trigger the biosynthesis of medicinally important bioactive metabolites, which influence the quality of raw plant material and finished products. Therefore, V. jatamansi and H. spicatum plants were undertaken and subjected to different levels of drought (no irrigation), heat (35 °C), and combined stresses for investigating their physiological and metabolic responses. Both the treatments (individually and in combination) reduced relative water content, photosynthesis, carboxylation efficiency, chlorophyll content, while increased intracellular CO2, malondialdehyde and H2O2 content in both the species. Transpiration and stomatal conductance increased under heat and reduced under drought stress as compared to control. Water use efficiency was found to be increased under drought, while reduced under heat stress. Protein, proline, carotenoid content and antioxidant enzymes activities (superoxide dismutase, peroxidise, catalase) initially increased and thereafter decreased during late stages of stress. Exposure of plants to combined stress was more detrimental than individual stress. In V. jatamansi, exposure to drought stress significantly (p < 0.05) increased valerenic acid content in all plant parts (1.0–6.9 fold) with maximum increase after 20 days of exposure, while under heat stress, valerenic acid content increased (1.0–1.2 fold) in belowground part of V. jatamansi, and decreased (1.1–1.3 fold) in aerial part as compared to control. In H. spicatum, exposure of individual heat stress for 25–30 days and combined stress for 5–15 days significantly (p < 0.05) increased linalool content to 6.2–6.5 fold and 8.3–19.6 fold, respectively, as compared to control. Higher accumulation of bioactive compounds after exposure to mild stress provides encouraging prospects for enhancing pharmaceutical properties of these Himalayan herbs.

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Abbreviations

CAT:

Catalase

CE:

Carboxylation efficiency

DMSO:

Dimethyl sulfoxide

H2O2 :

Hydrogen peroxide

HPLC:

High performance liquid chromatography

MDA:

Malondialdehyde

POD:

Peroxidise

PPFD:

Photosynthetic photon flux density

ROS:

Reactive oxygen species

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

RWC:

Relative water content

SMC:

Soil moisture content

SOD:

Superoxide dismutase

WUE:

Water use efficiency

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Acknowledgements

Authors acknowledge and thank Director, GBPNIHE for providing necessary facilities. Fellowship received by VP from Mountain Division (5th centre of GBPNIHE), MoEF&CC, New Delhi under Himalayan RA scheme is greatly acknowledged. VP also wishes to thank Late Prof. Alok Shukla, Department of Plant Physiology, Pantnagar University, for his initial guidance and support. The paper is dedicated to Late Dr. R.S. Rawal, former director, GBPNIHE. 

Funding

This work was partially supported by the Himalayan RA scheme of Mountain Division (5th centre of GBPNIHE), and NMHS, MoEF&CC, New Delhi sponsored project (file no. GBPNI/NMHS-2017-18/MG-19).

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Authors and Affiliations

  1. G. B. Pant National Institute of Himalayan Environment (GBPNIHE), Kosi-Katarmal, Almora, Uttarakhand, 263 643, India

    Veena Pandey, Deep C. Tiwari, Vibhash Dhyani, Indra D. Bhatt, Ranbeer S. Rawal & Shyamal K. Nandi

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  1. Veena Pandey
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  2. Deep C. Tiwari
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  3. Vibhash Dhyani
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  4. Indra D. Bhatt
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  5. Ranbeer S. Rawal
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  6. Shyamal K. Nandi
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Contributions

VP performed the experiments, analyzed data and wrote the manuscript. DCT and VD performed physiological experiments. IDB and RSR designed the experiments, interpreted the results and corrected the manuscript. SKN supervised the experiments.

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Correspondence to Indra D. Bhatt.

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Pandey, V., Tiwari, D.C., Dhyani, V. et al. Physiological and metabolic changes in two Himalayan medicinal herbs under drought, heat and combined stresses. Physiol Mol Biol Plants 27, 1523–1538 (2021). https://doi.org/10.1007/s12298-021-01027-w

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