Abstract
To enlarge the germplasm resource of Paulownia plants, we used colchicine to induce autotetraploid Paulownia tomentosa, as reported previously. Compared with its diploid progenitor, autotetraploid P. tomentosa exhibits better photosynthetic characteristics and higher stress resistance. However, the underlying mechanism for its predominant characteristics has not been determined at the proteome level. In this study, isobaric tag for relative and absolute quantitation coupled with liquid chromatography-tandem mass spectrometry was employed to compare proteomic changes between autotetraploid and diploid P. tomentosa. A total of 1427 proteins were identified in our study, of which 130 proteins were differentially expressed between autotetraploid and diploid P. tomentosa. Functional analysis of differentially expressed proteins revealed that photosynthesis-related proteins and stress-responsive proteins were significantly enriched among the differentially expressed proteins, suggesting they may be responsible for the photosynthetic characteristics and stress adaptability of autotetraploid P. tomentosa. The correlation analysis between transcriptome and proteome data revealed that only 15 (11.5%) of the differentially expressed proteins had corresponding differentially expressed unigenes between diploid and autotetraploid P. tomentosa. These results indicated that there was a limited correlation between the differentially expressed proteins and the previously reported differentially expressed unigenes. This work provides new clues to better understand the superior traits in autotetraploid P. tomentosa and lays a theoretical foundation for developing Paulownia breeding strategies in the future.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (Nos. 30271082, 30571496, U1204309), Natural Science Foundation of Henan Province (No. 162300410158) and Science and Technology Innovation Talents Project of Henan Province (No. 174200510001).
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12298_2017_447_MOESM11_ESM.tif
Supplementary material 11. Repeatability of the proteomic analysis between two biological replicates X-axis represents the difference of the quantitative ratios between biological replicate 1 and biological replicate 2 of the two samples. The right Y-axis represents the cumulative percentage between the proteins of a certain range and the quantitative proteins, while the left Y-axis represents the number of total protein in a certain range. (TIFF 9744 kb)
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Yan, L., Fan, G., Deng, M. et al. Comparative proteomic analysis of autotetraploid and diploid Paulownia tomentosa reveals proteins associated with superior photosynthetic characteristics and stress adaptability in autotetraploid Paulownia . Physiol Mol Biol Plants 23, 605–617 (2017). https://doi.org/10.1007/s12298-017-0447-6
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DOI: https://doi.org/10.1007/s12298-017-0447-6