Skip to main content
SpringerLink
Log in

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

  • Research Article
  • Published:
Physiology and Molecular Biology of Plants Aims and scope Submit manuscript

Abstract

A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at higher concentrations (20–30 %) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-dichloro phenoxy acitic acid

BAP:

6-Benzyl aminopurin

NAA:

α-Napthalene acitic acid

Kn:

Kinetin

CAT:

Catalase

POX:

Peroxidase

APX:

Ascorbate peroxidase

SOD:

Superoxide dismutase

MS:

Murashige and Skoog

ROS:

Reactive Oxygen Species

References

  • Aazami MA, Torabi M, Jalili E (2010) In vitro response of promising tomato genotypes for tolerance to osmotic stress. Afr J Biotechnol 9(26):4014–4017

    CAS  Google Scholar 

  • Abdel-Raheem AT, Ragab AR, Kasem ZA, Omar FD, Samera AM (2007) In vitro selection for tomato plants for drought tolerance via callus culture under polyethylene glycol (PEG) and mannitol treatments. Afr Crop Sci Conf Proc 8:2027–2032

    Google Scholar 

  • Aqeel-Ahmad MS, Javed F, Ashraf M (2007) Iso-osmotic effect of NaCl and PEG on growth, cations and free proline accumulation in callus tissue of two indica rice (Oryza sativa L.) genotypes. Plant Growth Regul 53:53–63

    Article  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Begum MK, Islam MO, Miah MAS, Hossain MA, Islam N (2011) Production of Somaclone In vitro for Drought Stress Tolerant Plantlet Selection in Sugarcane (Saccharum officinarum L.). Agriculturists 9(1&2):18–28

    Google Scholar 

  • Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in condition. Annal Biochem 161:559–566

    Article  CAS  Google Scholar 

  • Bhaskaran S, Smith RH, Newton RJ (1985) Physiological changes in cultured Sorgham cells in response to induced water stress 1. Free proline. Plant Physiol 79:239–248

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of micro gram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Bueno P, Piqueras A, Kurepa J, Savoure A, Veerbruggen N, Montegu MV, Inze D (1998) Expression of antioxidant enzymes in response to abscisic acid and high osmoticum in BY-2 cell cultures. Plant Sci 138(1):27–34

    Article  CAS  Google Scholar 

  • Chandlee JM, Scandalios JG (1984) Analysis of variants affecting the catalase development program in maiz scutellum Theor. Appl Genetics 69:71–77

    CAS  Google Scholar 

  • Davies KJA (1987) Protein damage and degradation by oxygen radicals. J Biol Chem 262:9895–9901

    PubMed  CAS  Google Scholar 

  • El-Haris MK, Barakat MN (1998) Evaluation of the in vitro selected drought tolerant wheat lines under drought stress conditions. Alex J Agric Res 43:293–302

    Google Scholar 

  • Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomar M, Senhaji NS (2006) Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiol Plant 29:95–102

    Article  Google Scholar 

  • Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomar M, Senhaji NS (2008) Growth, proline and ion accumulation in sugarcane callus cultures under drought-induced osmotic stress and its subsequent relief. Afri J Biotechnol 5:1488–1493

    Google Scholar 

  • Fridovich I (1986) Biological effects of superoxide radical. Arch Biochem Biophys 247:1–11

    Article  PubMed  CAS  Google Scholar 

  • Gangopadhyay G, Basu S, Mukherjee BB, Gupta S (1997) Effect of salt and osmotic shocks on unadapted and adapted callus lines of tobacco. Plant Cell Tiss Org Cult 49:45–52

    Article  CAS  Google Scholar 

  • Handa S, Handa AK, Hasegawa PM, Bressan RA (1986) Proline accumulation and the adaptation of cultured plant cells to water stress. Plant Physiol 80:938–945

    Article  PubMed  CAS  Google Scholar 

  • Hassan NS, Shaaban LD, El-Sayed AH, Seleem EE (2004) In Vitro Selection for Water Stress Tolerant Callus Line of Helianthus annus L Cv Myak. Int J Agric Biol http://www.ijab.org

  • Heath RL, Packer (1968) Photooxidation in isolated chloroplasts. Arch Biochem Biophys 125:189–198

    Article  PubMed  CAS  Google Scholar 

  • Jabeen FTZ (2007) Selection of drought tolerant cell lines in sugsrcane (Saccharum offcinarum Linn.) through cell and tissue culture technique. PhD. Thesis submitted to Gulbarga University Gulbarga Karnataka India

  • Jadhav YR, Garkar RM, Najan BR, Khade KK, Choudhary AN (1998) Nira (Co-86032) A new variety of sugarcane of Maharastra. J Maharastra agricuniv 23(1):10–12

    Google Scholar 

  • Kumar KB, Khan PA (1982) Peroxidase and Polyphenol oxidase in excised ragi (Elaucine coracona cv. 2020 leaves during senescence Indian. Expt Bot 20:412–416

    CAS  Google Scholar 

  • Kumar RR, Karjol K, Naik GR (2011) Effect of polyethylene glycol induced water stress on physiological and biochemical responses in Pigeon pea (Cajanus cajan L. Millsp.). RRST-Plant Physiol 3:148–152

    Google Scholar 

  • Larson RA (1988) The antioxidants of higher plants. Phytochemistry 27:969–978

    Article  CAS  Google Scholar 

  • Li L, van Stadan J (1998) Effects of plant growth regulators on the antioxidant systems in callus of two maiz cultivars subjected to water stress. Plant Growth Regul 24:55–66

    Article  Google Scholar 

  • Ming SY, ZhiDong Z, YaDong L, Wu L, Liu HG (2009) Effect of PEG stress on resistance of low bush blueberry callus. J Jilan Agric Univ 31(5):538–542

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Musa Y (2011) The use of Polyethylene glycol (PEG) as selection agent of callus and plantlets of some sugarcane varieties for drought tolerance. J Agrivigor 10(2):130–140

    Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplast. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Nath AK, Kumari S, Sharma DR (2005) In Vitro selection and chaterization of water stress tolerant cultures of bell pepper. Indian J Plant Physiol 10(1):14–19

    CAS  Google Scholar 

  • Patade VY, Bhargava S, Suprasanna P (2011) Salt and drought tolerance of sugarcane under iso-osmotic salt and water stress: growth osmolyte accumulation and antioxidant defense. J plant interact 6(4):275–282

    Google Scholar 

  • Patade VY, Bhargava S, Suprasanna P (2012) Effects of NaCl and iso-osmotic PEG stress on growth, Osmolytes accumulation and antioxidant defense in cultured sugarcane cells. Plant Cell Tissue Organ Cult 108:279–286

    Article  CAS  Google Scholar 

  • Prabha C, Bharti S (1980) Effect of ascorbic acid on proline accumulation in cowpea leaves under water stress conditions. Indian J Plant Physiol 23:317–318

    CAS  Google Scholar 

  • Sabbah S, Tal M (1990) Development of callus and suspension cultures of potato resistant to NaCl and mannitol and their response to stress. Plant Cell Tissue Organ Cult 21:119–124

    Article  CAS  Google Scholar 

  • Sadasivam S, Manickam A (1992) Biochemical methods for agricultural sciences, vol 80. Wiley Eastern Limited, New Delhi, pp 179–180

    Google Scholar 

  • Santos-Diaz N, Ochoa-Alejo N (1994) Effect of water stress on growth, osmotic potential and solute accumulation in cell cultures from chilli pepper (a mesophyte) and Creosote bush (a xerophytes). Plant Sci 96(1–2):21–29

    Article  CAS  Google Scholar 

  • Shah AH, Shah SH, Ahmad H, Swati ZA (2012) Adaptation to polyethylene stress maintains totipotency of cell lines of Oryza sativa L. CV Swat-1 for a longer period. Pak J Bot 44(1):313–316

    CAS  Google Scholar 

  • Siddeswar G, Kavi Kishor PB (1989) Plant regeneration from polyethylene glycol adapted callus of rice. Curr Sci 58:926–928

    Google Scholar 

  • Singh AK, Sharma DR (2008) In vitro screening and regeneration of water stress tolerant culture of tomato. Indian J Plant Physiol 13(1):33–38

    CAS  Google Scholar 

  • Srivastava DK, Gupta VK, Sharma DR (1995) In vitro selection and characterization of water stress tolerant callus cultures of tomato (Lycopersicon esculentum L. Mill cv. Ktl.). Indian J Plant Physiol 38:99–104

    Google Scholar 

  • Yaser F, Uzal O, Ozpay T (2010) Changes of lipidperoxidation andchlorophyll amount of greenbean genotypes underdrought stress. Afr J Agric Res 5(19):2705–2709

    Google Scholar 

Download references

Acknowledgements

The authors thank head department of Botany Gulbarga University Gulbarga for the facilities provided during the course of the investigation,

Author information

Authors and Affiliations

  1. Department of Botany, Gulbarga University, Gulbarga, India

    Srinath Rao & Jabeen FTZ

Authors
  1. Srinath Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jabeen FTZ
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Srinath Rao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rao, S., FTZ, J. In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.). Physiol Mol Biol Plants 19, 261–268 (2013). https://doi.org/10.1007/s12298-013-0162-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12298-013-0162-x

Keywords

Search

Navigation