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The lack of LHCII proteins modulates excitation energy partitioning and PSII charge recombination in Chlorina F2 mutant of barley

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Abstract

Analysis of the partitioning of absorbed light energy within PSII into fractions utilized by PSII photochemistry (ØPSII), thermally dissipated via ΔpH-and zeaxanthin-dependent energy quenching (ØNPQ) and constitutive non-photochemical energy losses (ØNO) was performed in wild type and F2 mutant of barley. The estimated energy partitioning of absorbed light to various pathways indicated that the fraction of ØPSII was slightly higher, while the proportion of thermally dissipated energy through ØNPQ was 38% lower in F2 mutant than in WT. In contrast, ØNO, i.e. the fraction of absorbed light energy dissipated by additional quenching mechanism(s) was 34% higher in F2 mutant. The increased proportion of ØNO correlated with narrowing the temperature gap (ΔT M) between S2/3QB− and S2QA− charge recombinations in F2 mutant as revealed by thermoluminescence measurements. We suggest that this would result in increased probability for an alternative non-radiative P680+QA− radical pair recombination pathway for energy dissipation within the reaction centre of PSII (reaction center quenching) and that this additional quenching mechanism might play an important role in photoprotection when the capacity for the primary, zeaxanthin-dependent non-photochemical quenching (ØNPQ) and state transitions pathways are restricted in the absence of LHCII polypeptides in F2 mutant.

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Abbreviations

LHCII:

the major Chl a/b pigment-protein complex associated with PSII

PSI:

photosystem I

PSII:

photosystem II

QA :

primary electron-accepting quinone in PSII reaction centres

QB :

secondary electron-accepting quinone in PSII reaction centers

NPQ:

non-photochemical quenching

qL:

photochemical quenching

TL:

thermoluminescence

TM :

temperature maximum of the TL emission peak

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Authors and Affiliations

  1. Department of Biology and The Biotron, University of Western Ontario, London, Ontario, Canada, N6A 5B7

    A. G. Ivanov, M. Krol & N. P. A. Huner

  2. Institute of Biophysics, Acad. G. Bonchev Str., Bl. 21, 1113, Sofia, Bulgaria

    Y. Zeinalov

  3. Jain Irrigation Systems Limited, Jain Hills, Jalgaon, 425 001, India

    P. V. Sane

Authors
  1. A. G. Ivanov
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  2. M. Krol
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  3. Y. Zeinalov
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  4. N. P. A. Huner
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  5. P. V. Sane
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Correspondence to N. P. A. Huner.

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Ivanov, A.G., Krol, M., Zeinalov, Y. et al. The lack of LHCII proteins modulates excitation energy partitioning and PSII charge recombination in Chlorina F2 mutant of barley. Physiol Mol Biol Plants 14, 205–215 (2008). https://doi.org/10.1007/s12298-008-0020-4

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  • DOI: https://doi.org/10.1007/s12298-008-0020-4

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