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A Pilot clinical study to evaluate the effect of Emblica officinalis extract (Amlamax™) on markers of systemic inflammation and dyslipidemia

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Abstract

Emblica officinalis Gaertn., commonly known as the Indian gooseberry or “Amla”, has been used as health food for centuries in India and other Asian countries. The biological effects of amla have been attributed to the antioxidant properties of the low-molecular weight hydrolysable tannins present in the fruit. Amlamax™ is a purified, standardized, dried extract of amla containing about 35% galloellagi tannins along with other hydrolysable tannins. Our earlier studies on rabbits showed significant reduction in total cholesterol and triglycerides as well as increase in HDL. The present study extends these results to human volunteers. Two doses of the extract were evaluated — 500 mg and 1000 mg per day for 6 months. Blood samples were collected at the 3rd and 6th months showed reduction in total and LDL cholesterols and enhancement of beneficial HDL cholesterol. In addition, blood CRP levels, a marker for inflammation, were also significantly reduced. Since dyslipidemia and inflammation are the two major components of cardiovascular diseases, the present results must be considered encouraging and indicate the potential of Amlamax™ in the management of heart diseases.

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References

  1. Satyavati GV, Raina MK, Sharma M. In: Medicinal plants of India, Vol. I, Indian Council of Medical Research, New Delhi 1976; p. 377–379.

    Google Scholar 

  2. Mishra M, Pathak UN, Khan AB. Emblica officinalis Gaertn and serum cholesterol level in experimental rabbits. Br J Exp Pathol 1981; 62(5): 526–528.

    PubMed  CAS  Google Scholar 

  3. Mathur R, Sharma A, Dixit VP, Varma M. Hypolipidaemic effect of fruit juice of Emblica officinalis in cholesterol-fed rabbits. J Ethnopharmacol 1996; 50(2): 61–68.

    Article  PubMed  CAS  Google Scholar 

  4. Thakur CP, Mandal K. Effect of Emblica officinalis on cholesterol-induced atherosclerosis in rabbits. Ind J Med Res 1984; 79: 142–146.

    CAS  Google Scholar 

  5. Annu J, Pandey M, Kapoor S, Saroja R. Effect of the Indian gooseberry (amla) on serum cholesterol levels in men aged 35–55 years. Eur J Clin Nutr 1988; 42: 939–944.

    Google Scholar 

  6. Anila L, Vijayalakshmi NR. Flavonoids from Emblica officinalis and Mangifera indica-effectiveness for dyslipidemia. J Ethnopharmacol 2002; 79:81–87.

    Article  PubMed  CAS  Google Scholar 

  7. Antony B, Merina B, Sheeba V, Mukkadan J. Effect of standardized Amla extract on atherosclerosis and dyslipidemia. Ind J Pharm Sci 2006; 68(4): 437–441.

    Article  CAS  Google Scholar 

  8. Bhattacharya SK, Bhattacharya A, Sairam K, Ghosal S. Effect of bioactive tannoid principles of Emblica officinalis on ischemia-reperfusion-induced oxidative stress in rat heart. Phytomedicine 2002; 9:171–174.

    Article  PubMed  CAS  Google Scholar 

  9. Ghosal S, Tripathi VK, Chauhan S. Active constituents of Emblica officinalis: Part I-The chemistry and anti-oxidative effects of two new hydrolysable tannins, Emblicanin A and B. Ind J Chem 1996; 35(B): 941–948.

    CAS  Google Scholar 

  10. Tariq M, Hussain SJ, Asif M, Jahan M. Protective effect of fruit extracts of Emblica officinalis (Gaertn) and Terminalia belerica (Roxb.) in experimental myocardial necrosis in rats. Ind J Exp Biol 1977; 15:485–486.

    CAS  Google Scholar 

  11. A composition to enhance HDL cholesterol, reduce inflammation and hyperglycemia and correct dysthyroidism in humans, and a method for its preparation. (Patent under processing)

  12. Antony B, Merina B, Sheeba V. Toxicity Studies of Amlamax™ — Purified Standardized Extract of Emblica Officinalis. Ind J Nat Prod 2007; 23(2):14–17.

    CAS  Google Scholar 

  13. Heart Disease and Stroke Statistics — 2006 Update. A report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee, Circulation, 2006, e85–e129; available from http://www.circulationaha.org

  14. Goldberg RJ, Spencer FA, Steg PG, Flather M, Montalescot G, Gurfinkel EP, et al. Increasing use of single and combination medical therapy in patients hospitalized for acute myocardial infarction in the 21st century. Arch Intern Med 2007; 167:1766–1773.

    Article  PubMed  Google Scholar 

  15. Ross R, Harker L. Hyperlipidemia and atherosclerosis. Science 1976; 193:1094–1100.

    Article  PubMed  CAS  Google Scholar 

  16. Libby P. Inflammation in atherosclerosis. Nature 2002; 420:868–874.

    Article  PubMed  CAS  Google Scholar 

  17. Pepys MB, Hirscfield GM. C-reactive protein: a critical update. J Clin Invest 2003; 111:1805–1812.

    PubMed  CAS  Google Scholar 

  18. Ridker PM, Glynn RJ, Hennekens CH. C-Reactive protein adds to the predictive value of total and HDL cholesterol in determining risk of first myocardial infarction. Circulation 1998;97:2007–2011.

    PubMed  CAS  Google Scholar 

  19. Lagrand WK, Visser CA, Hermens WT, Niessen HW, Verheugt FW, Wolbink GJ, Hack CE. C-Reactive protein as a cardiovascular risk factor: more than an epiphenomenon? [Review]. Circulation 1999;100:96–102.

    PubMed  CAS  Google Scholar 

  20. Libby P, Ridker PM. Novel inflammatory markers of coronary risk: theory versus practice [Editorial; comment]. Circulation 1999;100:1148–1150.

    PubMed  CAS  Google Scholar 

  21. Kluft C, de Maat MP, Gevers Leuven JA, Potter van Loon BJ, Mohrschladt MF. Statins and C-reactive protein [Letter]. Lancet 1999;353:1274.

    Article  PubMed  CAS  Google Scholar 

  22. Strandberg TE, Van Hanen H, Tikkanen MJ. Effect of statins on C-reactive protein in patients with coronary artery disease [Letter]. Lancet 1999;353:118–119.

    Article  PubMed  CAS  Google Scholar 

  23. Roeschlau P. In; Bergmeyer, H.U., Eds., Methods of Enzymatic Analysis, 2nd Edn., Academic Press, New York 1974; p. 1980.

    Google Scholar 

  24. Burstein M. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res 1970; 11: 583–595.

    PubMed  CAS  Google Scholar 

  25. Bucolo G, David H. Quantitative Determination of Serum Triglycerides by the Use of Enzymes. Clin Chem 1973; 19: 476–482.

    PubMed  CAS  Google Scholar 

  26. Frieldwald WT, Levi RI, Friedericksn DS. Estimation of the Concentration of Low- Density Lipoprotein Cholesterol in Plasma, Without Use of the Preparative Ultracentrifuge. Clin Chem 1972; 18: 499–502.

    Google Scholar 

  27. Binder CJ, Chang MK, Shaw PX, Miller YI, Hartvigsen K, Dewan A, Witztum JL. Innate and acquired immunity in atherosclerosis. Nat Med 2002; 8:1218–1226.

    Article  PubMed  CAS  Google Scholar 

  28. Abe I, Keshiwagi Y, Noguchi H, Tanake T, Ikeshiro Y, Kashiwada Y. Ellagitannins and hexahydroxydiphenoyl esters as inhibitors of vertebrate squalene epoxidase. J Nat Prod 2001; 64:1010–1014.

    Article  PubMed  CAS  Google Scholar 

  29. Cabana VG, Siegel JN, Sabesin SM. Effects of acute phase response on the concentration and density distribution of plasma lipids and apolipoproteins. J Lipid Res 1989; 30: 39–49.

    PubMed  CAS  Google Scholar 

  30. Sammalkorpi K, Valtonen V, Kerttula Y, Nikkila E, Taskinen MR. Changes in serum lipoprotein pattern induced by acute infections. Metabolism 1988; 37:859–865.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Research and Development Laboratory, Arjuna Natural Extracts Ltd., P.B No. 126, Bank Road, Alwaye, Kerala, 683101, India

    B. Antony, M. Benny & T. N. B. Kaimal

Authors
  1. B. Antony
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  2. M. Benny
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  3. T. N. B. Kaimal
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Correspondence to B. Antony.

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Antony, B., Benny, M. & Kaimal, T.N.B. A Pilot clinical study to evaluate the effect of Emblica officinalis extract (Amlamax™) on markers of systemic inflammation and dyslipidemia. Indian J Clin Biochem 23, 378–381 (2008). https://doi.org/10.1007/s12291-008-0083-6

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  • DOI: https://doi.org/10.1007/s12291-008-0083-6

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