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Evaluation of Bone Marrow Microvessel Density in Patients with Aplastic Anemia

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Bone marrow microenvironment plays a crucial role in the growth of hemopoietic cells and bone marrow function, which in turn depends on an intact microvasculature. Our study assesses the microvessel density (MVD) in the bone marrow of aplastic anemia (AA) patients, compares with MVD of controls and MVD among the different types of AA. Bone marrow specimens from 60 patients with AA and 17 controls were studied. There were 33 patients with non severe AA (NSAA), 12 patients with severe AA (SAA) and 15 patients with very severe AA (VSAA). MVD was calculated on sections stained immunohistochemically for CD34. The mean bone marrow MVD in AA group was 1.28 ± 0.36, being significantly lower than that in control group (6.80 ± 1.59, p < 0.001). MVD of SAA and NSAA patients were 1.16 ± 0.35 and 1.49 ± 0.27, respectively, being significantly different (p = 0.003). MVD of VSAA was 0.93 ± 0.25 and the difference with NSAA is significant, however there was no significant difference between SAA and VSAA. Bone marrow MVD is low in AA patients and is likely to have a role in pathophysiology of bone marrow failure. Proangiogenic agents together with specific therapy might accelerate the recovery of hematopoiesis in AA patients.

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Authors and Affiliations

  1. Department of Pathology, Armed Forces Medical College, Pune, 411040, India

    Venkatesan Somasundaram

  2. Brig Med, HQ Delhi Area, New Delhi, India

    Manvir Singh Tevatia

  3. Department of Pathology, Army College of Medical Sciences, Delhi Cantt., New Delhi, India

    Manvir Singh Tevatia

  4. Department of Pathology, All India Institute of Medical Sciences, Jodhpur, India

    Abhishek Purohit

  5. Department of Hematology, All India Institute of Medical Sciences, New Delhi, India

    Ankur Ahuja, Manoranjan Mahapatra, Seema Tyagi, Haraprasad Pati & Renu Saxena

Authors
  1. Venkatesan Somasundaram
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  2. Manvir Singh Tevatia
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  3. Abhishek Purohit
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  4. Ankur Ahuja
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  5. Manoranjan Mahapatra
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  6. Seema Tyagi
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  7. Haraprasad Pati
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  8. Renu Saxena
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Correspondence to Venkatesan Somasundaram.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was also approved by the institutional ethical committee.

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Informed consent was obtained from all individual participants included in the study.

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Somasundaram, V., Tevatia, M.S., Purohit, A. et al. Evaluation of Bone Marrow Microvessel Density in Patients with Aplastic Anemia. Indian J Hematol Blood Transfus 33, 169–174 (2017). https://doi.org/10.1007/s12288-016-0707-6

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  • DOI: https://doi.org/10.1007/s12288-016-0707-6

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