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Description of Ornithinimicrobium ciconiae sp. nov., and Ornithinimicrobium avium sp. nov., isolated from the faeces of the endangered and near-threatened birds

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Phenotypic and genomic analyses were performed to characterize two novel species, H23M54T and AMA3305T, isolated from the faeces of the Oriental stork (Ciconia boyciana) and the cinereous vulture (Aegypius monachus), respectively. Strains H23M54T and AMA3305T showed the highest similarities of 16S rRNA gene sequences and complete genome sequences with Ornithinimicrobium cavernae CFH 30183T (98.5% of 16S rRNA gene sequence similarity and 82.1% of average nucleotide identity, ANI) and O. pekingense DSM 21552T (98.5% of 16S rRNA gene sequence similarity and 82.3% of ANI), respectively. Both strains were Gram-stain-positive, obligate aerobes, non-motile, non-spore-forming, and coccoid- and rodshaped. Strain H23M54T grew optimally at 25–30°C and pH 8.0 and in the presence of 1.5–2% (wt/vol) NaCl, while strain AMA3305T grew optimally at 30°C and pH 7.0 and in the presence of 1–3% (wt/vol) NaCl. Both strains had iso-C15:0, iso-C16:0, and summed feature 9 (iso-C17:1ω9c and/or C16:0 10-methyl) as major cellular fatty acids. MK-8 (H4) was identified as the primary respiratory quinone in both strains. Strains H23M54T and AMA3305T possessed diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. Moreover, strains H23M54T and AMA3305T commonly contained ribose and glucose as major sugars and l-ornithine, l-alanine, glycine, and aspartic acid as major amino acids. The polyphasic taxonomic data indicate that strains H23M54T and AMA3305T represent novel species of the genus Ornithinimicrobium. We propose the names Ornithinimicrobium ciconiae sp. nov. and Ornithinimicrobium avium sp. nov. for strains H23M54T (= KCTC 49151T = JCM 33221T) and AMA3305T (= KCTC 49180T = JCM 32873T), respectively.

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Acknowledgements

We are grateful for the etymological advice given by Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel). This work was supported by grants from the Mid-Career Researcher Program (NRF-2020R1A2C3012797) through the National Research Foundation of Korea (NRF) and the National Institute of Biological Resources (NIBR201801106) funded by the Ministry of Environment of Korea (MOE). This work was also supported by the NRF grant funded by the Korean government (MSIT) (No. NRF-2018R1A5A1025077).

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  1. Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea

    So-Yeon Lee, Yun-Seok Jeong, Jeong Eun Han, Dong-Wook Hyun & Jin-Woo Bae

  2. Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea

    Hojun Sung, Pil Soo Kim, Hyun Sik Kim, Jae-Yun Lee, June-Young Lee, Euon Jung Tak & Jin-Woo Bae

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All sampling conducted in this study was approved by the Institutional Animal Care and Use Committee of Kyung Hee University (Permit number: KHUASP(SE)-18-048) and complied with the guidelines of the Committee.

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Lee, SY., Sung, H., Kim, P.S. et al. Description of Ornithinimicrobium ciconiae sp. nov., and Ornithinimicrobium avium sp. nov., isolated from the faeces of the endangered and near-threatened birds. J Microbiol. 59, 978–987 (2021). https://doi.org/10.1007/s12275-021-1323-1

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