Abstract
Raman spectroscopy is a promising tool for identifying microbial phenotypes based on single cell Raman spectra reflecting cellular biochemical biomolecules. Recent studies using Raman spectroscopy have mainly analyzed phenotypic changes caused by microbial interactions or stress responses (e.g., antibiotics) and evaluated the microbial activity or substrate specificity under a given experimental condition using stable isotopes. Lack of labelling and the nondestructive pretreatment and measurement process of Raman spectroscopy have also aided in the sorting of microbial cells with interesting phenotypes for subsequently conducting physiology experiments through cultivation or genome analysis. In this review, we provide an overview of the principles, advantages, and status of utilization of Raman spectroscopy for studies linking microbial phenotypes and functions. We expect Raman spectroscopy to become a next-generation phenotyping tool that will greatly contribute in enhancing our understanding of microbial functions in natural and engineered systems.
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11 March 2021
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Acknowledgments
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through The Strategic Initiative for Microbiomes in Agriculture and Food, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Project No. 918014-4), Research Program for Agricultural Science & Technology Development (Project No. PJ01419401), National Institute of Agricultural Sciences, Rural Development Administration and National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2019R1A4A1024764).
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Hong, JK., Kim, S.B., Lyou, E.S. et al. Microbial phenomics linking the phenotype to function: The potential of Raman spectroscopy. J Microbiol. 59, 249–258 (2021). https://doi.org/10.1007/s12275-021-0590-1
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DOI: https://doi.org/10.1007/s12275-021-0590-1