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Description of Pseudomonas asuensis sp. nov. from biological soil crusts in the Colorado plateau, United States of America

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A Gram-negative, aerobic, non spore-forming, non-motile, rod-shaped, yellow pigmented bacterium CP155-2T was isolated from a biological soil crusts sample collected in the Colorado plateau, USA and subjected to polyphasic taxonomic characterization. Strain CP155-2T contained summed feature 3 (C16:1 ω5c/C16:1 ω7c) and C18:1 ω7c as major fatty acids and diphosphatidylglycerol (DPG) along with phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as major polar lipids. Based on these characteristics CP155-2T was assigned to the genus Pseudomonas. Phylogenetic analysis based on 16S rRNA gene sequence further confirmed the affiliation of CP155-2T to the genus Pseudomonas and showed a 16S rRNA gene sequence similarity of less than 98.7% with already described species of the genus. Pseudomonas luteola, Pseudomonas zeshuii, and Pseudomonas duriflava were identified as the closest species of the genus Pseudomonas with 16S rRNA gene sequence similarities of 98.7%, 98.6%, and 96.9%, respectively. The values for DNA¨CDNA relatedness between CP155-2T and Pseudomonas luteola and Pseudomonas zeshuii were 23% and 14% respectively a value below the 70% threshold value, indicating that strain CP155-2T belongs to a novel taxon of the genus Pseudomonas lineage. The novel taxon status was strengthened by a number of phenotypic differences wherein CP155-2T was positive for oxidase, negative for gelatin hydrolysis, could utilize D-cellobiose, D-raffinose, L-rhamnose, D-sorbitol but not L-aspartic acid and L-glutamic acid. Based on the collective differences strain CP155–2T exhibited, it was identified as a novel species and the name Pseudomonas asuensis sp. nov. was proposed. The type strain of Pseudomonas asuensis sp. nov. is CP155–2T (DSM 17866T =ATCC BAA-1264T =JCM13501T =KCTC 32484T).

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  1. Gundlapally Sathyanarayana Reddy

    Present address: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad, 500 007, India

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  1. School of Life Sciences, Arizona State University, Main Campus, Tempe, AZ, 85287-4501, USA

    Gundlapally Sathyanarayana Reddy & Ferran Garcia-Pichel

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  1. Gundlapally Sathyanarayana Reddy
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Correspondence to Gundlapally Sathyanarayana Reddy.

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Supplemental material for this article may be found at http://www.springerlink.com/content/120956. GenBank/EMBL accession numbers for the 16S rRNA gene sequence of Pseudomonas asuensis sp. nov. (CP155-2T) is AJ871471.

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Reddy, G.S., Garcia-Pichel, F. Description of Pseudomonas asuensis sp. nov. from biological soil crusts in the Colorado plateau, United States of America. J Microbiol. 53, 6–13 (2015). https://doi.org/10.1007/s12275-015-4462-4

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