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Trans-membrane transport of n-octadecane by Pseudomonas sp. DG17

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

The trans-membrane transport of hydrocarbons is an important and complex aspect of the process of biodegradation of hydrocarbons by microorganisms. The mechanism of transport of 14C n-octadecane by Pseudomonas sp. DG17, an alkane-degrading bacterium, was studied by the addition of ATP inhibitors and different substrate concentrations. When the concentration of n-octadecane was higher than 4.54 μmol/L, the transport of 14C n-octadecane was driven by a facilitated passive mechanism following the intra/extra substrate concentration gradient. However, when the cells were grown with a low concentration of the substrate, the cellular accumulation of n-octadecane, an energy-dependent process, was dramatically decreased by the presence of ATP inhibitors, and n-octadecane accumulation continually increased against its concentration gradient. Furthermore, the presence of non-labeled alkanes blocked 14C n-octadecane transport only in the induced cells, and the trans-membrane transport of n-octadecane was specific with an apparent dissociation constant K t of 11.27 μmol/L and V max of 0.96 μmol/min/mg protein. The results indicated that the trans-membrane transport of n-octadecane by Pseudomonas sp. DG17 was related to the substrate concentration and ATP.

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  1. College of Water Sciences, Beijing Normal University, Beijing, 100875, P. R. China

    Fei Hua, Hong Qi Wang, Yi Li & Yi Cun Zhao

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  1. Fei Hua
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  2. Hong Qi Wang
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Correspondence to Hong Qi Wang.

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Hua, F., Wang, H.Q., Li, Y. et al. Trans-membrane transport of n-octadecane by Pseudomonas sp. DG17. J Microbiol. 51, 791–799 (2013). https://doi.org/10.1007/s12275-013-3259-6

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  • DOI: https://doi.org/10.1007/s12275-013-3259-6

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