Abstract
Full-length cDNAs of two different strains of Potato virus X (PVX-Kr and PVX-Mo) have been directly amplified by long template reverse transcription polymerase chain reaction (RT-PCR) using the 5′-end primer containing a SP6 or T7 RNA promoter sequence and the virus-specific 3′-end primer, and then constructed in plasmid vectors. Capped in vitro transcripts from cloned full-length cDNAs as well as those RT-PCR amplicons proved to be infectious systemically on tobacco plants. Symptom expression on tobacco plants from PVX-Mo transcripts was faster and severer than that from PVX-Kr. In replication stability test of transcripts derived from PVX clones, progeny viruses showed stable replication according to sequencing through passages. This highly infectious transcript system from the full-length cDNA clones for PVX can be useful for recombinant molecules for functional analysis of viral proteins in plant-virus interaction study as well as for expression of foreign protein in planta.
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Choi, S.H., Ryu, K.H. Generation of infectious transcripts from Korean strain and mild mottle strain of potato virus X. J Microbiol. 46, 502–507 (2008). https://doi.org/10.1007/s12275-008-0078-2
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DOI: https://doi.org/10.1007/s12275-008-0078-2