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Apigenin reduce lipoteichoic acid-induced inflammatory response in rat cardiomyoblast cells

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Abstract

Infective endocarditis is caused by Streptococcus sanguinis present in dental plaque, which can induce inflammatory responses in the endocardium. The present study depicts research on the properties of apigenin in embryonic mouse heart cells (H9c2) treated with lipoteichoic acid (LTA) obtained from S. sanguinis. Interleukin-1β and cyclooxygenase (COX)-2 expression were detected by reverse transcriptase polymerase chain reaction. In addition, western blot assays and immuno-fluorescence staining were used to assess translocation of nuclear factor kappa beta (NF-κB), degradation of IκB, as well as activity of the mitogen activated protein kinases: extracellular signal-regulated kinase (ERK)1/2, p38, and c-Jun N-terminal kinase (JNK). Effect of apigenin on cell viability was equally assessed in other experimental series. Our results showed that apigenin blocked activation of ERK, JNK, and p38 in cardiomyocytes treated with LTA in a dose-dependent fashion. Moreover, apigenin showed no cytotoxic effects; it blocked NF-κB translocation and IκB degradation. Our findings suggested that apigenin possessed potential value in the treatment of infectious endocarditis.

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Acknowledgments

We wish to express our thanks to the Dirección General de Asuntos del Personal Académico (DGAPA) PAPITT-IN 201816 for their financial support, and to Carmen Muñoz-Seca for editorial assistance.

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  1. Laboratorio de Bioquímica de la División de Estudios de Posgrado e Investigación de la Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Ciudad de México, DF, Mexico

    Gloria Gutiérrez-Venegas & Zeltzin González-Rosas

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  1. Gloria Gutiérrez-Venegas
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  2. Zeltzin González-Rosas
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Correspondence to Gloria Gutiérrez-Venegas.

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Gutiérrez-Venegas, G., González-Rosas, Z. Apigenin reduce lipoteichoic acid-induced inflammatory response in rat cardiomyoblast cells. Arch. Pharm. Res. 40, 240–249 (2017). https://doi.org/10.1007/s12272-016-0756-2

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