Abstract
A new lignan, (7′S,8S,8′S)-3,5′-dimethoxy-3′,4,9′-trihydroxy-7′,9-epoxy-8,8′-lignan, named vibruresinol (1), was isolated from the stems of Viburnum erosum by silica gel, ODS, and Sephadex LH-20 column chromatography. In addition, four other known lignans, (7′R,8S,8′S)-3,5′-dimethoxy-3′,4,8′,9′-tetrahydroxy-7′,9-epoxy-8,8′-lignan (2), (+)-syringaresinol (3), (+)-pinoresinol (4), and (+)-pinoresinol-4-O-β-d-glucopyranoside (5), and five known neolignans, herpetol (6), vibsanol (7), (−)-dehydrodiconiferyl alcohol (8), icariside E4 (9), and dihydrodehydrodiconiferyl alcohol (10), were isolated in the same manner. The chemical structures of the compounds were determined based on spectroscopic data including NMR, MS, and IR. All of the compounds described above were isolated from V. erosum for the first time. The isolated compounds 3, 4, and 6 were evaluated for neuroprotective activity on glutamate-induced cell death in HT22 cells and had EC50 values of 6.33 ± 1.22, 6.96 ± 0.65, and 9.15 ± 0.36 μM, respectively. Likewise, the same compounds had inhibitory activity on NO production in LPS-induced RAW 264.7 cells with IC50 values of 8.30 ± 1.56, 7.89 ± 1.22, and 9.32 ± 0.36 μM, respectively.
Similar content being viewed by others
References
Ahn, D.K. 1998. Illustrated book of Korean medicinal herbs, 355. Seoul: Kyohaksa.
Chun, J.M., M.S. Cheon, M. Park, A.Y. Lee, B.C. Moon, Y. Ji, and H.K. Kim. 2012. Inhibitory effects of an ethyl acetate fraction from Cephalonoplos segetum on inflammatory mediators from lipopolysaccharide-induced RAW 264.7 macrophages. Journal of Korean Society for Applied Biological Chemistry 55: 41–46.
Fukuyama, Y., M. Nakahara, H. Minami, and M. Kodama. 1996. Two new benzofuran-type lignans from the wood of Viburnum awabuki. Chemical & Pharmaceutical Bulletin 44: 1418–1420.
Hanawa, F., M. Shiro, and Y. Hayashi. 1997. Heartwood constituents of Betula maximowicziana. Phytochemistry 45: 589–595.
IUPAC. 2000. Nomenclature of lignans and neolignans. Pure and Applied Chemistry 72: 1493–1523.
Jeong, G.S., B. Li, D.S. Lee, E. Byun, D.K. Kang, H.S. Lee, and Y.C. Kim. 2007. Cytoprotective constituents of Alpinia katsumadai seeds against glutamate-induced oxidative injury in HT22 cells. Natural Product Sciences 13: 268–2721.
Kim, M.R., H.T. Moon, D.G. Lee, and E.R. Woo. 2007. A new lignan glycoside from the stem bark of Styrax japonica S. et Z. Archives of Pharmacal Research 30: 425–430.
Lee, S.H., G.S. Seo, and D.H. Sohn. 2004. Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochemical and Biophysical Research Communications 323: 125–132.
Lee, T.B. 2003. Coloured flora of Korea, vol. 2, 232. Seoul: Hyangmoonsa.
Lee, Y.J., N.S. Kim, H. Kim, J.M. Yi, S.M. Oh, O.S. Bang, and J. Lee. 2013. Cytotoxic and anti-inflammatory constituents from the seeds of Descurainia sophia. Archives of Pharmacal Research 36: 536–541.
Li, X.W., Z.T. Guo, Y. Zhao, Z. Zhao, and J.F. Hu. 2010. Chemical constituents from Saussurea cordifolia. Phytochemistry 71: 682–687.
Li, W., H. Shi, M. Wang, X. Wang, and X. Li. 2011. Chemical constituents of Viburnum erosum var. erosum and Viburnum odoratissimum var. awabuki. Chinese Pharmaceutical Journal 46: 1234–1237.
Makesbery, W.R. 1997. Oxydative stress hypothesis in Alzheimer’s disease. Free Radical Biology and Medicine 23: 134–147.
Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55–63.
Park, C., M. Zheng, E.A. Seo, K.B. Kwon, and D.G. Ryu. 2010a. Cytoprotective and anti-inflammatory effects of Nardostachys jatamansi water extract via expression of HO-1. Korean Journal of Oriental Physiology & Pathology 24: 624–629.
Park, J.H., S.W. Yeon, J.G. Cho, D.Y. Lee, Y.S. Kim, and N.I. Beak. 2010b. Lignans from silkworm droppings and their promotional activities on Heme Oxygenase-1 (HO-1). Journal of Korean Society for Applied Biological Chemistry 53: 734–739.
Simonian, N.A., and J.T. Coyle. 1996. Oxidative stress in neurodegenerative diseases. Annual Review of Pharmacology and Toxicology 36: 83–106.
Sumioka, H., L. Harinantenaina, K. Matsunami, H. Otsuka, M. Kawahata, and K. Yamaguchi. 2011. Linderolides A-F, eudesmane-type sesquiterpene lactones and linderoline, a germacrane-type sesquiterpene from the roots of Lindera strychnifolia and their inhibitory activity on NO production in RAW264.7 cells in vitro. Phytochemistry 72: 2165–2171.
Wang, W.S., X.C. Lan, H.B. Wu, Y.Z. Zhong, J. Li, Y. Liu, and C.C. Shao. 2012. Lignans from the flower buds of Magnolia liliflora Desr. Planta Medica 78: 141–147.
Woo, K.W., S.U. Choi, J.C. Park, and K.R. Lee. 2011. A new lignan glycoside from Juniperus rigida. Archives of Pharmacal Research 34: 2043–2049.
Wu, B., S. Wu, H. Qu, and Y. Cheng. 2008. Secondary metabolites from Viburnum erosum. Biochemical Systematics and Ecology 36: 817–819.
Xie, L.H., T. Akao, K. Hamasaki, T. Deyama, and M. Hattori. 2003. Biotransformation of pinoresinol diglucoside to mammalian lignans by human intestinal microflora, and isolation of Enterococcus faecalis strain PDG-1 responsible for the transformation of (+)-pinoresinol to (+)-lariciresinol. Chemical & Pharmaceutical Bulletin 51: 508–515.
Xu, B., P.P. Yang, P.L. Wang, H.L. Ling, and M. Chen. 2012. Study on the chemical constituents of Herpetospermum caudigerum. Zhongyaocai 35: 1080–1082.
Yang, C.P., G.J. Huang, H.C. Huang, Y.C. Chen, C.I. Chang, S.Y. Wang, H.S. Chang, Y.H. Tseng, S.C. Chien, and Y.H. Kuo. 2013a. The effect of the aerial part of Lindera akoensis on lipopolysaccharides (LPS)-induced nitric oxide production in RAW264.7 cells. International Journal of Molecular Sciences 14: 9168–9181.
Yang, E.J., J.Y. Lee, S.H. Park, T. Lee, and K.S. Song. 2013b. Neuroprotective effects of neolignans isolated from Magnoliae Cortex against glutamate-induced apoptotic stimuli in HT22 cells. Food and Chemical Toxicology 56: 304–312.
Yeo, H., Y.W. Chin, S.Y. Park, and J. Kim. 2004. Lignans of Rosa multiflora roots. Archives of Pharmacal Research 27: 287–290.
Zhonghuabencao Editorial Board. 1999. Zhonghuabencao [M], 552–553. Shanghai: Shanghai Scientific and Technical Press.
Acknowledgments
This research was supported by the (S2091482) project from the Academy and Research Institute funded Korea Small and Medium Business Administration.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
In, SJ., Seo, KH., Song, NY. et al. Lignans and neolignans from the stems of Vibrunum erosum and their neuroprotective and anti-inflammatory activity. Arch. Pharm. Res. 38, 26–34 (2015). https://doi.org/10.1007/s12272-014-0358-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12272-014-0358-9