Abstract
Cephalonoplos segetum has been used as an herbal remedy, and is considered to have anti-inflammatory potential. However, its biological mechanism in this treatment process remains unknown. Therefore, the anti-inflammatory activity of the ethyl acetate fraction of C. segetum extracts (CSE-EA), more active than C. segetum extracts (CSE) in murine macrophages, was investigated. Production levels of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) by lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were measured by ELISA. In addition, protein expression levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, and the phosphorylation of mitogenactivated protein kinases (MAPKs) in the LPS-induced macrophages were investigated by Western blotting. The CSE-EA (50, 100 or 200 μg/mL) significantly inhibited NO, PGE2, TNF-α, and IL-1β production in LPS-induced macrophages in a dose-dependent manner with 50% inhibitory concentration values of 80.4, 104.7, 91.3, and 46.7 μg/mL, respectively. Similarly, CSE-EA reduced protein expression of iNOS and COX-2 and led to the attenuated activation of kinases ERK1/2 and JNK in the macrophages. Results of the present study suggest that the anti-inflammatory effects of CSE-EA are likely due to the down-regulation of NO, PGE2 TNF-α, and IL-1β and the reduced expression of iNOS and COX-2 via suppression of MAPK signaling pathways in LPS-induced murine macrophages.
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M. S. Cheon’s present address: Biotechnology Examination Division, Chemistry and Biotechnology Examination Bureau, Korean Intellectual Property Office, Republic of Korea
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Chun, J.M., Cheon, M.S., Park, M. et al. Inhibitory effects of an ethyl acetate fraction from Cephalonoplos segetum on inflammatory mediators from lipopolysaccharide-induced RAW 264.7 macrophages. J Korean Soc Appl Biol Chem 55, 41–46 (2012). https://doi.org/10.1007/s13765-012-0007-2
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DOI: https://doi.org/10.1007/s13765-012-0007-2