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Hypoglycemic effects of Ganoderma lucidum polysaccharides in type 2 diabetic mice

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Abstract

Our aims were to investigate the hypoglycemic effects and mechanisms of action of Ganoderma lucidum polysaccharides (GLPs) administered for 7 days in type 2 diabetic mice. The mice were randomly divided into four groups (8 mice/group): normal control group, diabetic control group, low-dose GLP-treated diabetic group (50 mg/kg/d), and high-dose GLP-treated diabetic group (100 mg/kg/d). Diabetes was induced by streptozotocin injection and high-fat dietary feeding. At the end of the study, fasting serum glucose, insulin, body weight (BW) and epididymal white adipose tissue weight were measured. The hepatic mRNA levels of glycogen phosphorylase (GP), fructose-1,6-bisphosphatase (FBPase), phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) genes were determined by real-time polymerase chain reaction. Both doses of GLPs significantly decreased fasting serum glucose, insulin and epididymal fat/BW ratio compared with the diabetic control group (p < 0.05). The hepatic mRNA levels of GP, FBPase, PEPCK and G6Pase were significantly lower in both GLP-treated groups compared with the diabetic control group. Taken together, GLPs significantly decrease fasting serum glucose levels in type 2 diabetic mice in a dose-dependent manner. The decreases in fasting serum glucose levels may be associated with decreased mRNA expression levels of several key enzymes involved in gluconeogenesis and/or glycogenolysis.

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology (Ministry of Guangdong Province Joint Breeding Base), South China, Guangdong Institute of Microbiology, Guangzhou, 510070, China

    Chun Xiao, Qing-Ping Wu, Xiao-Bing Yang & Ju-Mei Zhang

  2. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Chun Xiao

  3. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China

    Chun Xiao

  4. Department of Toxicology, Center for Disease Control and Prevention of Guangdong Province, Guangzhou, 510020, China

    Wen Cai & Jian-Bin Tan

  5. Guangdong Institute of Microbiology, Xianlie Central Road 100, Guangzhou, 510070, China

    Qing-Ping Wu

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  1. Chun Xiao
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  2. Qing-Ping Wu
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  3. Wen Cai
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Correspondence to Qing-Ping Wu.

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These authors contributed equally to this work.

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Xiao, C., Wu, QP., Cai, W. et al. Hypoglycemic effects of Ganoderma lucidum polysaccharides in type 2 diabetic mice. Arch. Pharm. Res. 35, 1793–1801 (2012). https://doi.org/10.1007/s12272-012-1012-z

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  • DOI: https://doi.org/10.1007/s12272-012-1012-z

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