Abstract
Agro-herbal materials vary in prices and qualities depending on the origin and age and the differentiation is both scientific and public health issue. Here, we describe a metabolomics approach used to discriminate ginseng roots from different sources. Six different types of ginseng roots from China and Korea were analyzed by NMR-based metabolomics. Chinese Dangsam showed prominent differences and was easily differentiated. The difference was mainly due to the large signals in the sugar region. We further analyzed the metabolomics results in subgroups. Jeonra (Korean), Choongcheong (Korean), and Chinese ginseng in subgroup 1 could be easily differentiated by the first two principal components. The loading plot for PC1 showed that the Jeonra and Chinese ginseng roots were mainly separated by sugar signals and methyl signals but that they were reverse-correlated. A diffusion-ordered spectroscopy (DOSY) analysis showed that the methyl signals are from high molecular weight compounds and that the sugar signals are either from oligosaccharides or ginsenosides. In subgroup 2, composed of Korean Choongcheong ginseng at different ages, we were able to see age-dependent transitions in the score plot. We believe our approach can be applied to detecting the adulteration of ginseng root powders and other herbal products from different origins.
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Altieri, A. S., Hinton, D. P., and Byrd, R. A., Association of biomolecular systems via pulsed field gradient nmr self-diffusion measurements. J. Am. Chem. Soc., 117, 7566–7567 (1995).
Attele, A. S., Wu, J. A., and Yuan, C. S., Ginseng pharmacology: multiple constituents and multiple actions. Biochem. Pharmacol., 58, 1685–1693 (1999).
Brindle, J. T., Antti, H., Holmes, E., Tranter, G., Nicholson, J. K., Bethell, H. W., Clarke, S., Schofield, P. M., Mckilligin, E., Mosedale, D. E., and Grainger, D. J., Rapid and noninvasive diagnosis of the presence and severity of coronary heart disease using 1H-NMR-based metabonomics. Nat. Med., 8, 1439–1444 (2002).
Duarte, I., Barros, A., Belton, P. S., Righelato, R., Spraul, M., Humpfer, E., and Gil, A. M., High-resolution nuclear magnetic resonance spectroscopy and multivariate analysis for the characterization of beer. J. Agric. Food Chem., 50, 2475–2481 (2002).
Dunn, W. B., Bailey, N. J., and Johnson, H. E., Measuring the metabolome: Current analytical technologies. Analyst., 130, 606–625 (2005).
Lee, S. J., Ko, W. G., Kim, J. H., Sung, J. H., Moon, C. K., and Lee, B. H., Induction of apoptosis by a novel intestinal metabolite of ginseng saponin via cytochrome C-mediated activation of Caspase-3 protease. Biochem. Pharmacol., 60, 677–685 (2000).
Lucas, L. H., Otto, W. H., and Larive, C. K., The 2d-J-dosy experiment: Resolving diffusion coefficients in mixtures. J. Magn. Reson., 156, 138–145 (2002).
Moon, J., Yu, S. J., Kim, H. S., and Sohn, J., Induction of G(1) cell cycle arrest and P27(Kip1) increase by panaxydol isolated from Panax ginseng. Biochem. Pharmacol., 59, 1109–1116 (2000).
Murphy, L. L., Cadena, R. S., Chavez, D., and Ferraro, J. S., Effect of american ginseng (Pana quinquefolium) on male copulatory behavior in the rat. Physiol. Behav., 64, 445–450 (1998).
Nilsson, M., Duarte, I. F., Almeida, C., Delgadillo, I., Goodfellow, B. J., Gil, A. M., and Morris, G. A., High-resolution nmr and diffusion-ordered spectroscopy of port wine. J. Agric. Food Chem., 52, 3736–3743 (2004).
Robertson, D. G., Metabonomics in Toxicology: A Review. Toxicol. Sci., 85, 809–822 (2005).
Sengupta, S., Toh, S. A., Sellers, L. A., Skepper, J. N., Koolwijk, P., Leung, H. W., Yeung, H. W., Wong, R. N., Sasisekharan, R., and Fan, T. P., Modulating angiogenesis: The yin and the yang in ginseng. Circulation, 110, 1219–1225 (2004).
Sievenpiper, J. L., Arnason, J. T., Leiter, L. A., and Vuksan, V., Decreasing, null and increasing effects of eight popular types of ginseng on acute postprandial glycemic indices in healthy humans: The role of ginsenosides. J. Am. Coll. Nutr., 23, 248–258 (2004).
Simpson, A. J., Kingery, W. L., Spraul, M., Humpfer, E., Dvortsak, P., and Kerssebaum, R., Separation of structural components in soil organic matter by diffusion ordered spectroscopy. Environ. Sci. Technol., 35, 4421–4425 (2001).
Sumner, L. W., Mendes, P., and Dixon, R. A., Plant metabolomics: Large-scale phytochemistry in the functional genomics era. Phytochemistry, 62, 817–836 (2003).
Watkins, S. M. and German, J. B., Toward the implementation of metabolomic assessments of human health and nutrition. Curr. Opin. Biotechnol., 13, 512–516 (2002).
Weckwerth, W., Metabolomics in systems biology. Annu. Rev. Plant Biol., 54, 669–689 (2003).
Yang, S. Y., Kim, H. K., Lefeber, A. W., Erkelens, C., Angelova, N., Choi, Y. H., and Verpoorte, R., Application of two-dimensional nuclear magnetic resonance spectroscopy to quality control of ginseng commercial products. Planta Med., 72, 364–369 (2006).
Yun, T. K. and Choi, S. Y., Non-organ specific cancer prevention of ginseng: a prospective study in Korea. Int. J. Epidemiol., 27, 359–364 (1998).
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Jinho Kang and Seoyoung Lee contributed equally to this work.
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Kang, J., Lee, S., Kang, S. et al. NMR-based metabolomics approach for the differentiation of ginseng (Panax ginseng) roots from different origins. Arch. Pharm. Res. 31, 330–336 (2008). https://doi.org/10.1007/s12272-001-1160-2
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DOI: https://doi.org/10.1007/s12272-001-1160-2