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Coculture and Immobilization of Cellulolytic Bacteria for Enhanced Glucose Isomerase Production from Wheat Straw

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Abstract

Coculture and whole-cell immobilization have myriad industrial applications for enhancing enzyme production. Using pretreated wheat straw as the sole carbon source, improving glucose isomerase production and cell growth by synthetic bacterial consortia was investigated. Thirteen cocultures were constructed based on the performance and antagonistic activities of monocultures from six cellulolytic soil bacteria. The performance of monocultures immobilized with calcium alginate was also tested. Only five cocultures (A, B, C, G and J) exhibited cell growth and enzyme production synergies. The highest level of synergism (15.17 U/mL) was found in coculture J composed of Mycobacterium sp. MKAL3 (4.06 U/mL) and Stenotrophomonas sp. MKAL4 (3.37 U/mL) with a synergism degree of 2.04. The synergism was unique to growth on wheat straw as it was completely absent in xylose-grown cocultures. The wheat straw degradation synergism could rely on specific compounds released by the MKAL3 strain that promote the activity of the MKAL4 strain and vice versa. However, immobilized strains MKAL1, MKAL2, MKAL3, MKAL4 and MKAL5 improved glucose isomerase production in the wheat straw fermentation process at different sodium alginate concentrations. Immobilization studies of purified glucose isomerases for hydrolysis and saccharification of wheat straw are now being conducted.

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Acknowledgements

This project was supported by the Natural Science and Engineering Research Council of Canada (NSERC) Discovery Grant (RGPIN-2017-05366) to WQ.

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Authors and Affiliations

  1. Department of Biology, Lakehead University, Thunder Bay, Ontario, P7B 5E1, Canada

    Aristide Laurel Mokale Kognou, Chonlong Chio, Janak Raj Khatiwada, Sarita Shrestha, Xuantong Chen & Wensheng Qin

  2. School of Environment and Resources, Shanxi University, Taiyuan, 030006, China

    Yuen Zhu

  3. Department of Biochemistry, Faculty of Science, University of Douala, Douala, 24157, Cameroon

    Rosalie Anne Ngono Ngane

  4. Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies Cameroon, Yaoundé, 13033, Cameroon

    Gabriel Agbor Agbor

  5. Department of Chemistry, Lakehead University, Thunder Bay, ON, P7B 5E1, Canada

    Zi-Hua Jiang

  6. Department of Chemical and Biochemical Engineering, Western University, London, Ontario, N6A 5B9, Canada

    Chunbao Charles Xu

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  1. Aristide Laurel Mokale Kognou
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Contributions

WQ, CCX, ZHJ and ALMK contributed to the study conception and experimental design. The material preparation, data collection, curation data and analysis were carried out by ALMK. The manuscript draft was originally written by ALMK and edited by CC, JRK, SS, XC, YZ, RANN, GAA, ZHJ, CCX and WQ. The supervision and project administration were provided by WQ. All authors have read and approved the final version of the manuscript.

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Correspondence to Wensheng Qin.

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Kognou, A.L.M., Chio, C., Khatiwada, J.R. et al. Coculture and Immobilization of Cellulolytic Bacteria for Enhanced Glucose Isomerase Production from Wheat Straw. Biotechnol Bioproc E 28, 327–335 (2023). https://doi.org/10.1007/s12257-022-0254-y

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  • DOI: https://doi.org/10.1007/s12257-022-0254-y

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