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Kinetic and Thermodynamic Study of the Ultrasonic Acetone-pentane Fractional Precipitation of Paclitaxel from the Plant Cell Cultures of Taxus chinensis

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Abstract

In this study, the purification efficiency of paclitaxel was remarkably improved through ultrasonic acetone-pentane fractional precipitation. The time taken for precipitation was also shortened by a maximum of 192-fold (at 5°C, 180-250 W) with ultrasound as compared to that of the conventional method. The precipitation was completed within 30 min regardless of temperature (5-25°C), thereby improving the operating conditions (precipitation time and temperature) as compared to the conventional method. In addition, the rate constant in the fractional precipitation with ultrasound (80-250 W) was also increased by 8-to 13-fold (at 5°C) as compared to the conventional method. The standard enthalpy change and standard entropy change were negative, while the standard Gibbs free energy change was positive. These results indicate that the precipitation process was exothermic, irreversible, and non-spontaneous.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (NRF-2021R1A2C1003186).

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  1. Department of Chemical Engineering, Kongju National University, Cheonan, 31080, Korea

    Hye-Su Min & Jin-Hyun Kim

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  1. Hye-Su Min
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Min, HS., Kim, JH. Kinetic and Thermodynamic Study of the Ultrasonic Acetone-pentane Fractional Precipitation of Paclitaxel from the Plant Cell Cultures of Taxus chinensis. Biotechnol Bioproc E 26, 660–668 (2021). https://doi.org/10.1007/s12257-020-0345-6

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