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13C metabolite profiling to compare the central metabolic flux in two yeast strains

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Abstract

13C metabolite profiling to quantify the dynamic changes of central carbon metabolites was attempted using mass isotopomer distribution analysis in two yeast strains, Saccharomyces cerevisiae and Kluyveromyces marxianus. Mass and isotopomer balances of the intermediates were examined and calculated in both yeast species and central carbon metabolic fluxes were successfully determined. Metabolic fluxes of pentose phosphate pathway in K. marxianus were 1.66 times higher than S. cerevisiae. The flux difference was also supported by relatively high abundance of partially labeled fructose 6-phosphate and 3-phosphoglycerate as well as an increased concentration of labeled L-valine in K. marxianus. Metabolic flux analysis combined with dynamic metabolite profiling has provided better understanding in the central carbon metabolic pathways of two model organisms and can be applied as a method to analyze more complicated metabolic networks in other organisms.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Korea

    Joon-Young Jung, Dae-Kyun Im & Min-Kyu Oh

  2. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Korea

    Seok Hun Yun & Jinwon Lee

  3. CJ Research Institute of Biotechnology, Suwon, 16495, Korea

    Joon-Young Jung & Seok Hun Yun

Authors
  1. Joon-Young Jung
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  2. Seok Hun Yun
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  3. Dae-Kyun Im
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  4. Jinwon Lee
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  5. Min-Kyu Oh
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Correspondence to Jinwon Lee or Min-Kyu Oh.

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First two authors have contributed equally to this work.

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Jung, JY., Yun, S.H., Im, DK. et al. 13C metabolite profiling to compare the central metabolic flux in two yeast strains. Biotechnol Bioproc E 21, 814–822 (2016). https://doi.org/10.1007/s12257-016-0536-3

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  • DOI: https://doi.org/10.1007/s12257-016-0536-3

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