Abstract
Glucose dehydrogenase (GDH) locates in the cytoplasmic membrane of Gluconacetobacter xylinus oxidizes glucose to gluconic acid that decreases the conversion of glucose to bacterial cellulose (BC). In this study, a mutant of G. xylinus was generated by knocking-out the membrane bound GDH gene via homologous recombination of a defect GDH gene. The production of BC by G. xylinus mutant (GDH-KO strain) using glucose as a carbon source was investigated. Without the membrane bound GDH activity, the mutant strain still produces BC and increases glucose utilization efficiency for cellulose biosynthesis. In contrast, the wild-type strain oxidized a large fraction of glucose to gluconic acid that decreased the conversion yield of glucose to BC. Our results showed that the BC production from GDH-KO strain was about 40 and 230% higher than that of wild-type strain in static and shaken culture, respectively.
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Kuo, CH., Teng, HY. & Lee, CK. Knock-out of glucose dehydrogenase gene in Gluconacetobacter xylinus for bacterial cellulose production enhancement. Biotechnol Bioproc E 20, 18–25 (2015). https://doi.org/10.1007/s12257-014-0316-x
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DOI: https://doi.org/10.1007/s12257-014-0316-x