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Development of recombinantPseudomonas putida containing homologous styrene monooxygenase genes for the production of (S)-styrene oxide

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Abstract

Recently isolated,Pseudomonas putida SN1 grows on styrene as its sole carbon and energy source through successive oxidation of styrene by styrene monooxygenase (SMO), styrene oxide isomerase (SOI), and phenylacetaldehyde dehydrogenase. For the production of (S)-styrene oxide, two knockout mutants of SN1 were constructed, one lacking SOI and another lacking both SMO and SOI. These mutants were developed into whole-cell biocatalysts by transformation with a multicopy plasmid vector containing SMO genes (styAB) of the SN1. Neither of these self-cloned recombinants could grow on styrene, but both converted styrene into an enantiopure (S)-styrene oxide (e.e.>99%). Whole-cell SMO activity was higher in the recombinant constructed from the SOI-deleted mutant (130 U/g cdw) than in the other one (35 U/g cdw). However, the SMO activity of the former was about the same as that of the SOI-deleted SN1 possessing a single copy of thestyAB gene that was used as host. This indicates that the copy number ofstyAB genes is not rate-limiting on SMO catalysis by whole-cell SN1.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Pusan National University, 609-735, Busan, Korea

    Jong Wan Bae, Ju Hee Han, Mi So Park, Sun-Gu Lee & Sunghoon Park

  2. Institute for Environmental Technology and Industry, Pusan National University, 609-735, Busan, Korea

    Jong Wan Bae, Ju Hee Han, Mi So Park, Sun-Gu Lee & Sunghoon Park

  3. Department of Food Science and Technology, Kyungsung University, 608-736, Busan, Korea

    Eun Yeol Lee

  4. Division of Nano Science, Kook Min University, 120-750, Seoul, Korea

    Yong Joo Jeong

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  1. Jong Wan Bae
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  2. Ju Hee Han
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  4. Sun-Gu Lee
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Correspondence to Sunghoon Park.

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Bae, J.W., Han, J.H., Park, M.S. et al. Development of recombinantPseudomonas putida containing homologous styrene monooxygenase genes for the production of (S)-styrene oxide. Biotechnol Bioproc E 11, 530–537 (2006). https://doi.org/10.1007/BF02932079

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  • DOI: https://doi.org/10.1007/BF02932079

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