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Optimization of cultivation medium and fermentation parameters for lincomycin production by Streptomyces lincolnensis

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Abstract

Production of lincomycin by Streptomyces lincolnensis was studied by varying medium composition and environmental conditions. With the medium composition optimized by statistical experimental design (45 g/L soluble starch, 15 g/L sugar cane molasses, 13.33 g/L peptone water, 6.67 g/L NaNO3 and 4.0 g/L CaCO3), lincomycin production increased by 2 ∼ 3 fold compared to that obtained with un-optimized basal medium. Lincomycin production was further improved by optimizing culture conditions such as agitation speed, impeller type and pH under the optimized medium condition. The highest titer of 350 mg/L lincomycin was achieved from 240 h bioreactor culture. These results demonstrate that fermentation conditions for maximal lincomycin production by Streptomyces lincolnensis were optimized via biotechnological processes.

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Authors and Affiliations

  1. Research Center for Industrial Development of Biofood Materials, Jeonju, 561-759, Korea

    Yuri Lee & Yong-Seob Jeong

  2. Department of Food Science and Technology, Chonbuk National University, Jeonju, 561-759, Korea

    Yuri Lee & Yong-Seob Jeong

  3. Rural Development Administration, National Institute of Crop Science, Winter Cereal Division, Iksan, 570-080, Korea

    Mi-Ja Lee

  4. LED Agri-bio Fusion Technology Research Center, Chonbuk National University, Iksan, 570-752, Korea

    Yoon-E Choi

  5. College of Biomedical Science, Kangwon National University, Chuncheon, 200-701, Korea

    Gie-Taek Chun

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  1. Yuri Lee
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Correspondence to Yong-Seob Jeong.

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Lee, Y., Lee, MJ., Choi, YE. et al. Optimization of cultivation medium and fermentation parameters for lincomycin production by Streptomyces lincolnensis . Biotechnol Bioproc E 19, 1014–1021 (2014). https://doi.org/10.1007/s12257-014-0280-5

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  • DOI: https://doi.org/10.1007/s12257-014-0280-5

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