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Optimization of medium composition for improving biomass production of Lactobacillus plantarum Pi06 using the Taguchi array design and the Box-Behnken method

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Abstract

An immune-enhancing strain, Lactobacillus plantarum Pi06, isolated from a healthy infant was used for biomass production following optimization of the medium in shake-flask culture. Preliminary studies showed that commercial MRS medium and cultivation under static conditions generated higher biomass production than four other tested media with or without a shaking condition. The selected medium composition, consisting of glucose, yeast extract, soy peptone, ammonium citrate, and corn steep liquor, was further optimized using a systematic method that integrated the Taguchi array design and the Box-Behnken method. The response effects of these factors were first investigated using Taguchi design under an L 16 (45) array. The suggested medium composition, derived from Statistica 7.1 using the Taguchi design, was applied to cultivate cells and a biomass of 7.16 g dry cell weight (DCW)/L was obtained. Response surface methodology based on the Box-Behnken method for the three response variables of glucose, yeast extract, and corn steep liquor was then used to further increase the biomass level to 8.94 g DCW/L. The resulting optimum medium consisted of 35 g/L glucose, 35 g/L yeast extract, and 40 mL/L corn steep liquor. Compared with the initial medium, the biomass yield was improved from 4.31 to 8.94 g DCW/L, an enhancement of approximately 107%.

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Authors and Affiliations

  1. Department of Food Science and Technology, Hungkuang University, Taichung, 43302, Taiwan

    Chin-Fa Hwang, Jen-Han Chang, Cheng-Chih Tsai, Chien-Ku Lin & Hau-Yang Tsen

  2. Department of Chemical Engineering, I-Shou University, Kaohsiung, 84001, Taiwan

    Jer-Yiing Houng

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  1. Chin-Fa Hwang
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  2. Jen-Han Chang
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  3. Jer-Yiing Houng
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  4. Cheng-Chih Tsai
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Correspondence to Chin-Fa Hwang.

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Hwang, CF., Chang, JH., Houng, JY. et al. Optimization of medium composition for improving biomass production of Lactobacillus plantarum Pi06 using the Taguchi array design and the Box-Behnken method. Biotechnol Bioproc E 17, 827–834 (2012). https://doi.org/10.1007/s12257-012-0007-4

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  • DOI: https://doi.org/10.1007/s12257-012-0007-4

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