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Enhancement of taxol production from endophytic fungus Fusarium redolens

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Abstract

The optimization of taxol production by Fusarium redolens by one factor at a time (OFAT) approach led to production of 70 μg/L of taxol. With sucrose and NH4NO3 as the carbon and nitrogen sources and medium volume (V m ) to flask volume (V f ) ratio of 0.2, a greater taxol production was attained. NH4NO3, MgSO4↔7H2O and NaOAc at 6.25, 0.63, and 1.25 g/L, were the significant factors for attaining the highest taxol production. The optimization of culture variables led to the production of taxol from 66 to 198 μg/L, which is three fold higher than that in the unoptimized medium. Current study results suggested the success of Response Surface Methodology in enhancing the production of fungal taxol.

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Authors and Affiliations

  1. Department of Biotechnology, Thapar University, Patiala, 147-004, India

    Sanjog Garyali, Anil Kumar & M. Sudhakara Reddy

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  1. Sanjog Garyali
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  2. Anil Kumar
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  3. M. Sudhakara Reddy
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Correspondence to M. Sudhakara Reddy.

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Garyali, S., Kumar, A. & Reddy, M.S. Enhancement of taxol production from endophytic fungus Fusarium redolens . Biotechnol Bioproc E 19, 908–915 (2014). https://doi.org/10.1007/s12257-014-0160-z

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  • DOI: https://doi.org/10.1007/s12257-014-0160-z

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