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Ethylene promotes mycelial growth and ganoderic acid biosynthesis in Ganoderma lucidum

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Abstract

Objective

To investigate the effects of ethylene, in the form of ethephon (2-chloroethylphosphonic acid), on mycelial growth and ganoderic acid (GA) accumulation in the higher basidiomycete Ganoderma lucidum.

Results

Treatment with both 10 and 15 mM ethephon enhanced the growth of G. lucidum on solid CYM plates and in CYM liquid medium. After optimization using response surface methodology, GA reached 33 mg/g dry cell weight (DW), an increase of 90 %, compared with the control. Lanosterol and squalene contents were 31 and 2.4 μg/g DW, being increased by 1.2- and 0.6-fold, respectively, in response to ethephon. Additionally, the transcriptional levels of hydroxymethylglutaryl-CoA reductase, squalene synthase and oxidosqualene cyclase were up-regulated by 2.6-, 4.3- and 3.8-fold, respectively, compared with the control group.

Conclusions

This approach provides an efficient strategy for improving GA accumulation in G. lucidum, with potential future applications.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Project No. 31300039), the Natural Science Foundation of Jiangsu Province of China (Project No. BK20130675), Chinese National Key Technology R&D Program (Project No. 2013BAD20B05), and the Youth Sci-Tech Innovation Fund, Nanjing Agricultural University (Project No. KJ2013029).

Supplementary information

Supplementary Table 1—Design matrix of the central composite design and response.

Supplementary Table 2—Primer sets used for real time PCR.

Supplementary Fig. 1—Ethylene production in G. lucidum with ethephon treatment.

Author information

Authors and Affiliations

  1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, No.1 Weigang, Nanjing, 210095, Jiangsu, People’s Republic of China

    Guang Zhang, Ang Ren, Fengli Wu, Hanshou Yu, Liang Shi & Mingwen Zhao

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  1. Guang Zhang
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  2. Ang Ren
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  3. Fengli Wu
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  4. Hanshou Yu
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  5. Liang Shi
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  6. Mingwen Zhao
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Corresponding author

Correspondence to Liang Shi.

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Zhang, G., Ren, A., Wu, F. et al. Ethylene promotes mycelial growth and ganoderic acid biosynthesis in Ganoderma lucidum . Biotechnol Lett 39, 269–275 (2017). https://doi.org/10.1007/s10529-016-2238-5

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  • DOI: https://doi.org/10.1007/s10529-016-2238-5

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