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Optimization of operational conditions for adipate ester synthesis in a stirred tank reactor

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Abstract

Esterification of adipic acid and oleyl alcohol in a solvent-free system featuring a stirred tank reactor containing commercially immobilized Candida antarctica lipase B was performed. The process was carried out using an artificial neural network (ANN) trained by the Levenberg-Marquardt (LM) algorithm. The effects of four operative variables, temperature, time, amount of enzyme, and impeller speed, on the reaction yield were studied. By examining different ANN configurations, the best network was found to consist of seven hidden nodes using a hyperbolic tangent sigmoid transfer function. The values of the coefficient of determination (R2) and root mean squared error (RMSE) between the actual and predicted responses were determined to be 1 and 0.0058178 for training and 0.99467 and 0.622540 for the testing datasets, respectively. These results imply that the developed model was capable of predicting the esterification yield. The operative variables affected the yield, and their order of contribution was as follows: time > amount of enzyme > temperature > impeller speed. A high percentage of yield (95.7%) was obtained using a low level of enzyme (2.5% w/w), and the temperature, time, and impeller speed were 66.5°C, 354 min (about 6 h), and 500 rpm, respectively. A simple protocol for efficient substrate conversion in a solvent-free system evidenced by high enzyme stability is indicative of successful ester synthesis.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia

    Naz Chaibakhsh, Mohd Basyaruddin Abdul Rahman & Mahiran Basri

  2. Structural Biology Research Center, Malaysia Genome Institute, MTDCUKM Smart Technology Centre, UKM Bangi, 43600, Bangi, Selangor, Malaysia

    Mohd Basyaruddin Abdul Rahman

  3. Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran

    Farzaneh Vahabzadeh

  4. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia

    Suraini Abd-Aziz

  5. Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia

    Abu Bakar Salleh

Authors
  1. Naz Chaibakhsh
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  2. Mohd Basyaruddin Abdul Rahman
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  3. Farzaneh Vahabzadeh
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  4. Suraini Abd-Aziz
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  5. Mahiran Basri
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  6. Abu Bakar Salleh
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Correspondence to Mohd Basyaruddin Abdul Rahman.

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Chaibakhsh, N., Rahman, M.B.A., Vahabzadeh, F. et al. Optimization of operational conditions for adipate ester synthesis in a stirred tank reactor. Biotechnol Bioproc E 15, 846–853 (2010). https://doi.org/10.1007/s12257-010-0001-7

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  • DOI: https://doi.org/10.1007/s12257-010-0001-7

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