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Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system

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  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60°C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

    Naz Chaibakhsh, Mohd Basyaruddin Abdul Rahman & Mahiran Basri

  2. Structural Biology Research Center, Malaysia Genome Institute, MTDC-UKM, Smart Technology Centre, 43600 UKM Bangi, Selangor, Malaysia

    Mohd Basyaruddin Abdul Rahman

  3. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

    Suraini Abd-Aziz

  4. Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

    Abu Bakar Salleh & Raja Noor Zaliha Raja Abdul Rahman

Authors
  1. Naz Chaibakhsh
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  2. Mohd Basyaruddin Abdul Rahman
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  3. Suraini Abd-Aziz
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  4. Mahiran Basri
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  5. Abu Bakar Salleh
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  6. Raja Noor Zaliha Raja Abdul Rahman
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Correspondence to Mohd Basyaruddin Abdul Rahman.

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Chaibakhsh, N., Abdul Rahman, M.B., Abd-Aziz, S. et al. Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system. J Ind Microbiol Biotechnol 36, 1149–1155 (2009). https://doi.org/10.1007/s10295-009-0596-x

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  • DOI: https://doi.org/10.1007/s10295-009-0596-x

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