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Excellent laundry detergent compatibility and high dehairing ability of the Bacillus pumilus CBS alkaline proteinase (SAPB)

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Abstract

The newly Tunisian soil-isolated bacterium, producing the alkaline proteinase termed SAPB that was already purified and characterized [1], was assigned as Bacillus pumilus CBS strain on the basis of biochemical properties and 16S rRNA gene sequencing. The maximum protease activity recorded after 24 h of incubation in an optimized medium at 37°C was 6,500 U/mL in shaking flask culture and 25,000 U/mL in fermentor. SAPB showed excellent stability and compatibility in laundry detergent retaining more than 98% of its initial activity after pre-incubation for 1 h at 40°C with Det, followed by OMO (97%), Dinol (94%), and Dixan (93%). Examination of various stained cloth pieces exhibited a remarkable efficiency in the removal of blood and chocolate stains. More interestingly, SAPB demonstrated powerful dehairing capabilities of hair removal from skin with minimal damage on the collagen and a nearly complete feather-degradation. Likewise, Bacillus pumilus CBS effectively degraded feather-meal (98.5%), chicken feather (92%), goat hair (80%), and bovine hair (68%) whereas sheep wool under went less degradation. Keratin-degradation resulted in sulfhdryl group formation (0.95∼3.91 μM).

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Authors and Affiliations

  1. Laboratoire d’Enzymes et de Métabolites des Procaryotes (LEMP), Centre de Biotechnologie de Sfax (CBS), B.P 1177, Route Sidi Mansour Km 6, 3018, Sfax, Tunisia

    Bassem Jaouadi, Mamdouh Ben Ali, Ezzedine Ben Messaoud, Belgacem Naili & Samir Bejar

  2. Unité Enzymes et Bioconversion (UEB), Ecole Nationale d’Ingénieurs de Sfax (ENIS), B.P 1173, Route Soukra Km 3, 3038, Sfax, Tunisia

    Semia Ellouz-Chaabouni

  3. Laboratoire des Bioprocédés (LBP), Centre de Biotechnologie de Sfax (CBS), B.P 1177, Route Sidi Mansour Km 6, 3018, Sfax, Tunisia

    Abdelhafidh Dhouib

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  1. Bassem Jaouadi
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  2. Semia Ellouz-Chaabouni
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  3. Mamdouh Ben Ali
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  4. Ezzedine Ben Messaoud
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  5. Belgacem Naili
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  6. Abdelhafidh Dhouib
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  7. Samir Bejar
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Correspondence to Samir Bejar.

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Jaouadi, B., Ellouz-Chaabouni, S., Ali, M.B. et al. Excellent laundry detergent compatibility and high dehairing ability of the Bacillus pumilus CBS alkaline proteinase (SAPB). Biotechnol Bioproc E 14, 503–512 (2009). https://doi.org/10.1007/s12257-008-0244-8

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  • DOI: https://doi.org/10.1007/s12257-008-0244-8

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