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Optimization, production, and partial characterization of an alkalophilic amylase produced by sponge associated marine bacterium Halobacterium salinarum MMD047

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An Erratum to this article was published on 01 December 2009

Abstract

An endosymbiont Halobacterium salinarum MMD047, which could produce high yields of amylase, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1% sucrose. The enzyme was found to be produced constitutively even in the absence of starch. The optimum temperature and pH for the enzyme production was 40°C and 8.0, respectively. The enzyme exhibited maximum activity in pH range of 6∼10 with an optimum pH of 9.0. The enzyme was stable at 40°C and the enzyme activity decreased dramatically above 50°C. Based on the present findings, the enzyme was characterized as relatively heat sensitive and alkalophilic amylase which can be developed for extensive industrial applications.

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Authors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620024, India

    S. Shanmughapriya, Joseph Selvin, R. Gandhimathi, T. Bastin Baskar, Aseer Manilal & S. Sujith

  2. Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, India

    G. Seghal Kiran

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  1. S. Shanmughapriya
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  2. G. Seghal Kiran
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  3. Joseph Selvin
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Correspondence to Joseph Selvin.

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An erratum to this article can be found online at http://dx.doi.org/10.1007/s12257-009-1003-1.

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Shanmughapriya, S., Seghal Kiran, G., Selvin, J. et al. Optimization, production, and partial characterization of an alkalophilic amylase produced by sponge associated marine bacterium Halobacterium salinarum MMD047. Biotechnol Bioproc E 14, 67–75 (2009). https://doi.org/10.1007/s12257-008-0060-1

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