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High Expression of PhospholipaseD2 Induced by Hypoxia Promotes Proliferation of Colon Cancer Cells through Activating NF- κ Bp65 Signaling Pathway

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Pathology & Oncology Research

Abstract

Hypoxia is a typical feature of colon cancer occurrence and progression. We have reported that high expression and activity of PhospholipaseD2 (PLD2) induced by hypoxia in colon cancer cells. In order to further investigate the role of PLD2 in colon cancer under hypoxic conditions. MTT assay was used to detect the proliferation of human colon cancer cells (SW480 and SW620) under hypoxic conditions by decrease the PLD2 gene expression or inhibit the activity of PLD2. Expression level of p-P65/T-P65 and Cyclin D1 were detected in those cells treated as above through using western blot and RT-PCR analysis. Effect of NF-Bp65 inhibitor (BAY-117082) on the proliferation and expression level of Cyclin D1 and PLD2 of colon cancer cells under hypoxic conditions were further analysised. As a result, decreased the expression of PLD2 or inhibited the activity of PLD2 leaded to the proliferation of hypoxia colon cancer cells reduced, and along with the expression level of p-P65/T-P65 and Cyclin D1 reduced. However, inhibition the expression level of p-P65/T-P65 lead to the proliferation and expression of Cyclin D1 in those hypoxia colon cancer cells also reduced. In vivo growth decreased in response to PLD2 and NF-Bp65 inhibition. Our study indicates that high expression of PLD2 induced by hypoxia promotes the proliferation of colon cancer cells, and it may elevate the expression level of Cyclin D1 through activating NF-Bp65 signaling pathway. Inhibition of the PLD2 expression may provide a new clue for treatment for colon cancer.

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Acknowledgements

This work was supported by the Natural Science Foundation of Shanxi Province, No. 201701D121092. The authors gratefully thank Laboratory Research Center of Shanxi cancer Hospital for providing equipment support.

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Authors and Affiliations

  1. Department of Anorectal Surgery, Shanxi Cancer Hospital, Taiyuan, 030013, People’s Republic of China

    Maoxi Liu & Bo Jiang

  2. Department of Anorectal Surgery, Xijing Hospital, Xian, 710032, People’s Republic of China

    Kunli Du

  3. Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Xingye Wu

Authors
  1. Maoxi Liu
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  2. Kunli Du
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  3. Bo Jiang
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  4. Xingye Wu
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Contributions

Bo Jiang and Xingye Wu conceived and designed the experiments; Maoxi Liu and Kunli Du performed the experiments; Maoxi Liu analyzed the data and wrote the paper.

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Correspondence to Bo Jiang or Xingye Wu.

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The authors declare no conflict of interest.

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Liu, M., Du, K., Jiang, B. et al. High Expression of PhospholipaseD2 Induced by Hypoxia Promotes Proliferation of Colon Cancer Cells through Activating NF- κ Bp65 Signaling Pathway. Pathol. Oncol. Res. 26, 281–290 (2020). https://doi.org/10.1007/s12253-018-0429-1

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  • DOI: https://doi.org/10.1007/s12253-018-0429-1

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